Protein Info for GFF3488 in Sphingobium sp. HT1-2

Annotation: Hydrolase, alpha/beta fold family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF00561: Abhydrolase_1" amino acids 61 to 304 (244 residues), 124.3 bits, see alignment E=9.9e-40 PF12146: Hydrolase_4" amino acids 62 to 291 (230 residues), 33.8 bits, see alignment E=3.5e-12 PF12697: Abhydrolase_6" amino acids 63 to 309 (247 residues), 67.6 bits, see alignment E=3.9e-22

Best Hits

KEGG orthology group: None (inferred from 80% identity to mrd:Mrad2831_4681)

Predicted SEED Role

"Alpha/beta hydrolase fold precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>GFF3488 Hydrolase, alpha/beta fold family (Sphingobium sp. HT1-2)
MKKLVISMLLATAGIAASADAIAAPARAVATQAAAATVHYRSATIDGVKMFYREAGPADG
PVVLLLHGFPTSSHMFRNLIPLLADRYHVIAPDYPGFGQSDAPDHRQFTYSFAHYADMVD
TLMGKVGARRYAMYLMDYGAPVGYRLALKHPERVSALIVQNGNAYEEGLGAFWDPIKTYW
QSGSAKDREAQAWLVGLDATKFQYTDGVRDLSRISPDNWVQDQALLDRPGNKDIQLDLFY
DYRTNVPLYPAFQAFFRERKPPTLIVWGKNDKIFPEPGAHPYKRDLPDAEMHILDTGHFA
LEDKLDEMAPLIRDFLDRKVAVR