Protein Info for GFF3480 in Xanthobacter sp. DMC5

Annotation: putative acyl-CoA dehydrogenase fadE25

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 26 to 122 (97 residues), 56.5 bits, E=5.4e-19
• PF00441: Acyl-CoA_dh_1
  • amino acids 280 to 419 (140 residues), 25 bits, E=2.7e-09

Best Hits

KEGG orthology group: None (inferred from 94% identity to xau:Xaut_3186)

Predicted SEED Role

"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)

MetaCyc Pathways

• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Fatty acid metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2

Use Curated BLAST to search for 1.3.99.2

Protein Sequence (467 amino acids)

>GFF3480 putative acyl-CoA dehydrogenase fadE25 (Xanthobacter sp. DMC5)
MSETKMWGKEEFWGLGFEWDPQWLLSAEQKDLQAKLIALCETTLRANAVESDANYIYPRK
NFEALATLGLLSLNVPKELGGLGQNHVCAAMVVETIARYGCPSTAMCYTMHLGAVAAALL
RHHDNPLLTDILKRLDKDVLIGTLSYSDPETGSHFWYPISSKAEAHGDGWKVTKKASWTT
SGGFADWYIVQTTSPGFSGDYSDLSCFLVTKDEVKANPSEWDGLGLRGNQSGPIVVENAV
LPKEALVGPIGDGAKSNDEVVDPFFLTCSSACWNGISLAVIDIAKRHTTRKTHNDVGMRV
ADYPTIQDYVGEAIMDTNSCRAFDYQMAQALDSVTNNCDWSIHKDIDALPRSSLLHWLWQ
VKFEAAKNVAHVVDKMLHATGGTGFRPALQIERYLRDGKAGWVMGPTNEVLRQFVGKAAL
LGFGSLDYWNKSINERVLNNEIKKMDEAEKRAFAEKLLAELQPAKVA