Protein Info for GFF3470 in Sphingobium sp. HT1-2

Annotation: ATP synthase F0 sector subunit b

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 164 transmembrane" amino acids 15 to 34 (20 residues), see Phobius details PF02326: YMF19" amino acids 3 to 58 (56 residues), 27.7 bits, see alignment E=4e-10 PF00430: ATP-synt_B" amino acids 15 to 141 (127 residues), 55.7 bits, see alignment E=5.7e-19

Best Hits

Swiss-Prot: 31% identical to ATPF2_BRADU: ATP synthase subunit b 2 (atpF2) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02109, F-type H+-transporting ATPase subunit b [EC: 3.6.3.14] (inferred from 88% identity to sch:Sphch_0348)

Predicted SEED Role

"ATP synthase B' chain (EC 3.6.3.14)" in subsystem F0F1-type ATP synthase (EC 3.6.3.14)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (164 amino acids)

>GFF3470 ATP synthase F0 sector subunit b (Sphingobium sp. HT1-2)
MPQIAQIADTYASQIFWLLLTFGFVFFVIGRGMVPKVQATADARDAKISGDLDAAKAAFA
RADEAEADYRVRDAESRTAAQATLAKAKADAAKASEAKLAAADAEIQDKIGAAEARIKTA
TDAAMAQIETVAADAARDMVVRISGVEASDEAARNAVKAALAHG