Protein Info for PS417_17745 in Pseudomonas simiae WCS417

Annotation: gluconolactonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF20067: SSL_N
  • amino acids 46 to 90 (45 residues), 54 bits, 2.5e-18
• PF08450: SGL
  • amino acids 85 to 274 (190 residues), 53.5 bits, E=5.3e-18
• PF03088: Str_synth
  • amino acids 153 to 241 (89 residues), 74.1 bits, E=1.6e-24

Best Hits

KEGG orthology group: None (inferred from 92% identity to pfs:PFLU3998)

Predicted SEED Role

"Strictosidine synthase precursor (EC 4.3.3.2)" (EC 4.3.3.2)

MetaCyc Pathways

• secologanin and strictosidine biosynthesis (1/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Indole and ipecac alkaloid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.3.2

Sequence Analysis Tools

See A0A1N7UK50 at UniProt or InterPro

Protein Sequence (366 amino acids)

>PS417_17745 gluconolactonase (Pseudomonas simiae WCS417)
MSTRPKKLRHLLFVLFLAVIAFLLLMPTKVQPVNWTPPKAPSMKSGPYAENQRLKGVQRI
GAQDIAGPEALLLDTQGYLISGLHDGRIIRTSPDSRSLEVLVNTGGRPLGLALHPDGRLI
VADGIKGLLALDAKRQLTTLSTSANGVPFGFTDDVTVDASGRYAYFSDASSRWGYGQDGE
AVIEHGGDGRLLRYDFSNGTTEVLLDQLQFANGVALGPDENFVLVNETGAYRISRYWLKG
ERAGTHDLFLDNLPGLPDNLSFNGQDRFWVALYSPRNPLLDRFAGYPSLRKVMVRALMVV
PKPIEHKAFVLGLDTEGKVIANLQDGSAGNYSPITTAREYGNWLYLGSLTSTSMARLPLD
LALAGE