Protein Info for GFF3465 in Sphingobium sp. HT1-2

Annotation: Excinuclease ABC subunit C

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 662 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR00194: excinuclease ABC subunit C" amino acids 60 to 640 (581 residues), 542.4 bits, see alignment E=7.3e-167 PF01541: GIY-YIG" amino acids 69 to 144 (76 residues), 33.7 bits, see alignment E=1.3e-11 PF02151: UVR" amino acids 260 to 287 (28 residues), 25.8 bits, see alignment (E = 2.4e-09) PF22920: UvrC_RNaseH" amino acids 303 to 417 (115 residues), 136.2 bits, see alignment E=1.7e-43 PF08459: UvrC_RNaseH_dom" amino acids 433 to 594 (162 residues), 169.3 bits, see alignment E=2.2e-53 PF14520: HHH_5" amino acids 609 to 657 (49 residues), 40.6 bits, see alignment 1e-13 PF12826: HHH_2" amino acids 613 to 661 (49 residues), 27.4 bits, see alignment 1e-09

Best Hits

Swiss-Prot: 72% identical to UVRC_SPHAL: UvrABC system protein C (uvrC) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K03703, excinuclease ABC subunit C (inferred from 91% identity to sch:Sphch_0350)

Predicted SEED Role

"Excinuclease ABC subunit C" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (662 amino acids)

>GFF3465 Excinuclease ABC subunit C (Sphingobium sp. HT1-2)
MNAGVGRPLFHTAIAIRAGAAISAAMSQPQSPDRFNEDKATYTVTGSQPDIDAGVEAIRT
TLKTLPTRPGVYRMQDARGDVLYVGKARALKNRVANYTQVDRLPNRLRRMVAQTRSMTIV
TTNSEAEALLLEAQLIKRYRPPYNVLLRDDKSFPFILLREDHDFPRVQKHRGARKYKGRY
YGPFASAGSVTRTINALQKLFLLRSCTDSFFANRSRPCLLYQIKRCSAPCVDRIDTAGYA
ELVQDAQDFLGGKSTAVQQKLGAAMEAAAEALDFEQAAMIRDRLKALTFIQGSQAINAEG
LGDADIFALAEKGGSMCIQAFFIRGGQNWGHRSFFPVHTNDVATEEVLESFMAQFYEEVP
PPKLILTDREPAECELMALALSERLGSKVRIEVPQRGERTRLIKQAQRNAVEALDRRLAE
TTTQAKILDEMVEAFGLDGVPDRIEIYDNSHIQGAHALGAMVVAGPEGFRKNAYRKFNMK
NPDISNDDFAMMREMFERRFGRAAREDPDRDGGEWPDLVLIDGGKGQLSAARAMLEDMGI
EDVCMIGIAKGPHHGRDGREVFHLMDGREVSFPINHPLLFYMQRLRDEAHRFAIGAHRAK
RSKAITVSSLDEVPGIGPARKKALLMHFGTARAVRDAALEDLQRAPGVSKAVAQQVYDYF
HG