Protein Info for GFF3461 in Sphingobium sp. HT1-2

Annotation: Acetyl-CoA hydrolase (EC 3.1.2.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02550: AcetylCoA_hydro
  • amino acids 11 to 222 (212 residues), 90.3 bits, E=1.7e-29
• TIGR03458: succinate CoA transferase
  • amino acids 13 to 502 (490 residues), 770.8 bits, E=2.7e-236
• PF13336: AcetylCoA_hyd_C
  • amino acids 332 to 469 (138 residues), 142.4 bits, E=1.1e-45

Best Hits

Swiss-Prot: 62% identical to SCACT_ACEAC: Succinyl-CoA:acetate CoA-transferase from Acetobacter aceti

KEGG orthology group: None (inferred from 87% identity to sch:Sphch_1868)

MetaCyc: 62% identical to succinyl-CoA:acetate CoA-transferase monomer (Acetobacter aceti)
RXN-8807 [EC: 2.8.3.18]

Predicted SEED Role

"Acetyl-CoA hydrolase (EC 3.1.2.1)" (EC 3.1.2.1)

MetaCyc Pathways

• pyruvate fermentation to acetate V (3/3 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• acetate formation from acetyl-CoA (succinate) (1/1 steps found)
• pyruvate fermentation to acetate VI (2/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• succinate fermentation to butanoate (2/7 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.1

Use Curated BLAST to search for 2.8.3.18 or 3.1.2.1

Protein Sequence (505 amino acids)

>GFF3461 Acetyl-CoA hydrolase (EC 3.1.2.1) (Sphingobium sp. HT1-2)
MASDRIGHAGFAAKRRSAQDAAALIADGMTVGMSGFTGSGYPKAVPLALADRITAAHSAG
DPFRVKVWTGASTGPELDGALAKADGVEFRLPYNSDPIARERINRGEMQYFDMHLSQVAP
MAWQGFLGELDVAVVEVTGITPDGLLIPSASVGNNKTWLDRAKGIILEVNSWQNAALEGM
HDIYYGTALPPSRVPIPLTRADDRIGEPYFRVDPAKVLAVVETDSPDRNAPFTPPDDSAR
AIAGHLLEFLGHEVKMGRIPASLLPLQSGVGNIANAVLTGLIDAPFDNMVSYTEVIQDGM
LDLLESGKLRMASATAFSLSPEAAARLNADMARFRNKMILRPQEISNHPELVRRLGCIAM
NGLIEADIYGNVNSTHVMGSRIQNGIGGSGDFARNAYLSIFMTPSTAKQGKISAIVPHAS
HVDHINQDVQVIVTEQGLADLRGLAPRQRAELIINNCAHPDYRPMLKDYFRRAMQGSYGL
QSPMLPAEAFAWHQRFIDTGTMMPG