Protein Info for GFF3459 in Xanthobacter sp. DMC5

Annotation: 1-deoxy-D-xylulose-5-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 733 PF00676: E1_dh" amino acids 37 to 345 (309 residues), 205.3 bits, see alignment E=1.7e-64 PF02779: Transket_pyr" amino acids 399 to 572 (174 residues), 96.1 bits, see alignment E=2.9e-31 PF02780: Transketolase_C" amino acids 589 to 708 (120 residues), 73 bits, see alignment E=3.3e-24

Best Hits

KEGG orthology group: K11381, 2-oxoisovalerate dehydrogenase E1 component [EC: 1.2.4.4] (inferred from 70% identity to put:PT7_2792)

Predicted SEED Role

"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) / Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (733 amino acids)

>GFF3459 1-deoxy-D-xylulose-5-phosphate synthase (Xanthobacter sp. DMC5)
MPETIALQPAAPWVELKSTEADWAAADPALLKELLAQMALIRAFEEKVLELAGEGLVHGP
AHSSIGQEGGAAGSIVGLRPTDQVNGSHRGHHQFLAKALAYVAPGLDPTAPLSDDTHALL
HRSLAEILGLEPGFGHGRGGSMHLRWGEAGAIGTNAIVGGGVPLAAGAAFAHKRAGTGDV
AISYFGDGAVNIGAVLETMNLAAAWDLPLLFFIENNGYAVATTVEEATRETRLSGRGTGF
GIPSFKVDGMDPLAVHLATQEALKIMRSGHGPAVIEADVYRYFHQNGPLPGSAFGYRTRE
EEAAWRARDPIKRTGAALVARGVLTQDDLDALTERAQAAMADAAARLVEPVGNKRRVKPD
MWPDVSFVDEGIRGDLSELSGKRAEEEATYRGTLSERKFVDAIADVLHRRFETDERFVIM
GEDIHKLKGGTNGATRGLAAAFPDRCLGTPISEGAFAGLAGGIAMDRRYRPIIEFMYPDF
MWVAADPVFNQIGKARHMFGADVPVPLVLRTKIAMGTGYGSQHSLDPAGVFATSPGWRIV
APSTPFDYVGLMNAALACEDPVLVIEHVDLYQTSGAIPEGDLDYIIPFGKAKVVRPGGEL
TILTYSAMVHRSREAVEELGLDAEIIDLRSLDRAGLDWDTIGESIRKTNNVLIVEQGSLS
TSYGGFLADEIQRRYLDWLDQPIQRVTGKIASPTISKVLERAAFAGKEEVIAGLAVTMAA
KGMPLPGLPGAAR