Protein Info for GFF3458 in Sphingobium sp. HT1-2

Annotation: 3-isopropylmalate dehydrogenase (EC 1.1.1.85)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00169: 3-isopropylmalate dehydrogenase
  • amino acids 3 to 341 (339 residues), 448.3 bits, E=9.1e-139
• PF00180: Iso_dh
  • amino acids 3 to 343 (341 residues), 442.4 bits, E=7e-137

Best Hits

Swiss-Prot: 73% identical to LEU3_NOVAD: 3-isopropylmalate dehydrogenase (leuB) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K00052, 3-isopropylmalate dehydrogenase [EC: 1.1.1.85] (inferred from 92% identity to sjp:SJA_C1-22380)

MetaCyc: 56% identical to isopropylmalate dehydrogenase 3 (Arabidopsis thaliana col)
3-isopropylmalate dehydrogenase. [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]; 1.1.1.85 [EC: 1.1.1.85]

Predicted SEED Role

"3-isopropylmalate dehydrogenase (EC 1.1.1.85)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 1.1.1.85)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• butanol and isobutanol biosynthesis (engineered) (2/8 steps found)
• aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.85

Protein Sequence (347 amino acids)

>GFF3458 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (Sphingobium sp. HT1-2)
MLIALFPGDGIGPEIVAQAKRVLDALAIDGLTYEEGLVGGSAYKAVGHPLPPETLDIARR
ADAILFGAVGDPDCDSLERHLRPEQAILGLRKELGLFSNLRPAKVFPELAHESALKPEVA
GSIDLLIVRETNGDVYFGEKGMRTTTDGLREGYDIMSYNEAEVRRIAHAGFQAARARRGK
LCSVDKANVLETSQLWRDVVIEVSAEYPDVALTHMYVDNAAMQLVRNPGQFDVIVTGNMF
GDILSDQASMCVGSIGMLASATLNGSGQGLYEPIHGSAPDIAGQGKANPLATILSAAMML
RYSLNLPEPADRIEAAVAKALADGARSPDLGGTMNTVEMGDAVLAAL