Protein Info for GFF3457 in Xanthobacter sp. DMC5

Annotation: Shikimate dehydrogenase (NADP(+))

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08501: Shikimate_dh_N
  • amino acids 22 to 105 (84 residues), 81.4 bits, E=4.5e-27
• PF01488: Shikimate_DH
  • amino acids 135 to 182 (48 residues), 30.5 bits, 3.5e-11

Best Hits

Swiss-Prot: 41% identical to AROE_RUBXD: Shikimate dehydrogenase (NADP(+)) (aroE) from Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)

KEGG orthology group: K00014, shikimate dehydrogenase [EC: 1.1.1.25] (inferred from 82% identity to xau:Xaut_1616)

MetaCyc: 45% identical to 5-amino-3-dehydroquinate dehydrogenase (Amycolatopsis mediterranei U32)
1.1.1.-

Predicted SEED Role

"Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 1.1.1.25)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• superpathway of chorismate metabolism (41/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.25

Use Curated BLAST to search for 1.1.1.25

Protein Sequence (279 amino acids)

>GFF3457 Shikimate dehydrogenase (NADP(+)) (Xanthobacter sp. DMC5)
MTASTTAPNPREISGLTKVYGILADPIHHVKTPQMLNALMAREGRDGVMVPFHVSSEELP
RLVDGLKAMKSLGGFVVTVPHKTAIVDLLDEVSDSARRIGACNVVRREADGRLIGDMLDG
KGFVGGLKAAGIDPKGKGVYLAGAGGAANAIAFAFVEAGVARLTIANRTRAKAEDLAARL
ADAFPGAPVEIGTADPSGHDIVANATSLGLKAGDALPLDASRLTSDQIVAEVIMQPEETA
ILAAAKAKGCRTHPGKPMLVCQLDLMADFLGMRSLGAAS