Protein Info for GFF3443 in Xanthobacter sp. DMC5

Annotation: Putative phosphonates utilization ATP-binding protein PhnK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 259 transmembrane" amino acids 202 to 220 (19 residues), see Phobius details TIGR02323: phosphonate C-P lyase system protein PhnK" amino acids 6 to 257 (252 residues), 375.7 bits, see alignment E=4.7e-117 PF00005: ABC_tran" amino acids 24 to 181 (158 residues), 106.4 bits, see alignment E=1.9e-34

Best Hits

Swiss-Prot: 66% identical to PHNK_ECOLI: Putative phosphonates utilization ATP-binding protein PhnK (phnK) from Escherichia coli (strain K12)

KEGG orthology group: K05781, putative phosphonate transport system ATP-binding protein (inferred from 93% identity to xau:Xaut_1312)

MetaCyc: 66% identical to ATP-binding cassette protein PhnK (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Phosphonates transport ATP-binding protein PhnK" in subsystem Alkylphosphonate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (259 amino acids)

>GFF3443 Putative phosphonates utilization ATP-binding protein PhnK (Xanthobacter sp. DMC5)
MTTDTPLLTAQGLAKYYGHRLGCRDVDLTLHEGEVLAIVGESGSGKSTLLGLLSTELAPT
AGRVAYRMRDGETRDLASLSEAERRLLLRTDWGFVRQHAEQGLRMAVSAGGNVGERLMAV
GWRHYGDIRSEATDWLSRVEIAAERIDDDPRTFSGGMRQRLQIARNLVTRPRLVFMDEPT
SGLDVSVQARLLDLIRQLVEEFGLAVVIVTHDLAVARLLSHRIMVMKSGRVIEQGLTDQV
LDDPREPYTQLLVSSVLAA