Protein Info for GFF3442 in Xanthobacter sp. DMC5

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 351 PF06007: PhnJ" amino acids 16 to 286 (271 residues), 487.2 bits, see alignment E=5.4e-151

Best Hits

Swiss-Prot: 83% identical to PHNJ_RHIME: Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase (phnJ) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K06163, PhnJ protein (inferred from 94% identity to xau:Xaut_1313)

MetaCyc: 83% identical to alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase (Sinorhizobium meliloti 1021)
RXN-17959 [EC: 4.7.1.1]

Predicted SEED Role

"PhnJ protein" in subsystem Alkylphosphonate utilization

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.7.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (351 amino acids)

>GFF3442 hypothetical protein (Xanthobacter sp. DMC5)
MNAPTTAPASSMPAITYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGT
GGVQVTAAILGADDVLKVIDQGSDDTTNAVAIRTFFETTAGVATTTRTQDATIIQTRHRI
PEAGLKAGQTIVFQVPIPEPLRFLEPRETETRRLHGLADYGLMQVKLYEDIARHGHIATT
YAYPVKVEGRYVMDPSPIPKFDNPKMNDSPALQLFGAGREKRIYAIPPYTQVVSLDFEDH
PFKVQAFKEPCALCGATGVYLDEVILDDKGGRMFVCSDTDHCATRQADGHTGTMSAPYVA
QSAPLTPSPSLARGEGGNPAGIPSPLAGEGGSRSSRETGEGFATGESGEAR