Protein Info for PS417_01755 in Pseudomonas simiae WCS417

Annotation: proline-specific permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 469 transmembrane" amino acids 19 to 38 (20 residues), see Phobius details amino acids 44 to 63 (20 residues), see Phobius details amino acids 91 to 119 (29 residues), see Phobius details amino acids 125 to 143 (19 residues), see Phobius details amino acids 155 to 177 (23 residues), see Phobius details amino acids 200 to 223 (24 residues), see Phobius details amino acids 243 to 263 (21 residues), see Phobius details amino acids 277 to 302 (26 residues), see Phobius details amino acids 333 to 354 (22 residues), see Phobius details amino acids 360 to 382 (23 residues), see Phobius details amino acids 403 to 425 (23 residues), see Phobius details amino acids 431 to 449 (19 residues), see Phobius details PF00324: AA_permease" amino acids 16 to 451 (436 residues), 386 bits, see alignment E=2.5e-119 PF13520: AA_permease_2" amino acids 19 to 443 (425 residues), 116.7 bits, see alignment E=1.3e-37

Best Hits

Swiss-Prot: 61% identical to PROY_ECOLI: Proline-specific permease ProY (proY) from Escherichia coli (strain K12)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 98% identity to pfs:PFLU0368)

MetaCyc: 47% identical to phenylalanine:H+ symporter PheP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-56

Predicted SEED Role

"Histidine transport protein (permease)" in subsystem Histidine Degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TX33 at UniProt or InterPro

Protein Sequence (469 amino acids)

>PS417_01755 proline-specific permease (Pseudomonas simiae WCS417)
MQQQEKGLKRGLSARHIRFMALGSAIGTGLFYGSASAIQMAGPAVLLAYLIGGAAVFMVM
RALGEMAVHNPVAGSFGQYASTYLGPMAGFILGWTYAFEMIIVCLADVTAFGIYMGFWFP
DVARWVWVLGIVLLIGGLNLCNVKVFGEMEFWLSLLKVGAIVAMILGGFGIMLFGIHSAG
ETQASGLSNLWAHGGFMPNGIGGLIASFAVVMFAFGGIEIIGITAGEAKDPQRVIPKAIN
AVPLRILLFYVLTLFVLMAIYPWPQIGSQGSPFVQIFSNLGIGSAATILNIVVISAAVSA
INSDIFGAGRMMYGLAQQGQAPKGFAQLSKQGVPWMTVVVMGAALLGGVVLNYLIPENVF
LVIASIATFATVWVWLMILFTQVAMRRSMTKEQVAELKFPVPFWPYAPAAAIVFMLFVFG
VLGYFPDTQAALLVGAVWIVLLVVAYLLWVKPSAGQAAKVHYDAALSHR