Protein Info for GFF3431 in Sphingobium sp. HT1-2

Annotation: Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K00697, alpha,alpha-trehalose-phosphate synthase (UDP-forming) [EC: 2.4.1.15] (inferred from 87% identity to sjp:SJA_C1-14720)

MetaCyc: 37% identical to alpha, alpha-trehalose-phosphate synthase [ADP-forming] monomer (Mycobacterium tuberculosis H37Rv)
RXN-761 [EC: 2.4.1.347]

Predicted SEED Role

"Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15)" in subsystem Trehalose Biosynthesis (EC 2.4.1.15)

MetaCyc Pathways

trehalose biosynthesis I (2/2 steps found)
trehalose biosynthesis II (2/2 steps found)
glycogen biosynthesis III (from α-maltose 1-phosphate) (4/8 steps found)

KEGG Metabolic Maps

Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.15 or 2.4.1.347

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (468 amino acids)

>GFF3431 Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15) (Sphingobium sp. HT1-2)
MSRLIVISNRVSRPNGAANQGGLAVALAQALRESRGIWVGWSGGTTDNFTGHIGFADDDG
VKTATIDLEEQDVDEYYNGYANKTLWPLFHYRIDLAEYARDFEGGYNRVNQRFADTASPL
IEPEDIIWVQDYHMIPLGQMLRDRGHQNRMGFFLHIPWPPTRLLVSLPHHTKLVSTLFAY
DVIGFHTEEWLESFRHYVEREMGGTVDGDFVTVGGRTIQAVACPIGINAEEFKQAAASDA
AVEMHRQVRASLQDRSLIVGVDRLDYSKGLEERFNGYARFLKDHAEHHRRVVLAQIAPPS
RGEVESYQQIRATLDSLAGRINGEYSDVDWTPIRYVNQGYPRDKLAGIYRSARIGLVTPL
RDGMNLVAKEYVAAQNPDDPGVLILSRFAGAAMQLKDALLINPYSPEEMSDAIDRALAMP
LDERKRRWRAMMDSVEQQDISWWRSAFTDRLAATRQEEPEAEPEPATE