Protein Info for GFF3430 in Sphingobium sp. HT1-2

Annotation: Glucoamylase (EC 3.2.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 88% identity to sch:Sphch_0399)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (574 amino acids)

>GFF3430 Glucoamylase (EC 3.2.1.3) (Sphingobium sp. HT1-2)
MVWACVPRVDGDPVFSALLDNKHWDSKEARGFWAIELENCVAIEQHYIRNTPILITRQSD
GQGNAIEIFDFCPRHKHKGRMYRPVAFARIVRPAAGSPRIRVRLRPTTSWGKENVPVTYG
SNHIRMVLSYMAMRISTNAPIGLISQESWFRLESDMHFFMGPDEGFSDALRPAIERMLDD
TILEWQLWVRGLAIPPEWQEAVIRSAITLKLCQHEETGAIVAALTTSIPEHADSGRNWDY
RYCWVRDAYYTVEALNRLGALDVLETYLVYLRNIVDGAKGGHIQPLYDVRGNATLHEWEA
AHLPGYRGMGPVRVGNAAYEQIQHDAYGQIVLSSVQGFIDRRLLRMAGHADFEALEAVGE
RAWQVYDQPDAGLWELRTRAHVHSYSAVMCWAACDRLAHAATALDLPLRAAHWENRAETM
RARIIESAWRADSKAISANFEDDARDASLLQLLDLRFLTADDPMFTGTLEALEKDLRRGN
DMLRYSAPDDFGEPVTAFNVCTFWLIEALHRTGRTEEARELFEEMLSRRTAAGLLSEDID
PHNGELWGNYPQTYSLVGIINCAVLLSKPWSVMR