Protein Info for GFF3425 in Sphingobium sp. HT1-2

Annotation: DNA topoisomerase IV subunit A (EC 5.99.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 775 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 15 to 775 (761 residues), 808.8 bits, see alignment E=2.1e-247 PF00521: DNA_topoisoIV" amino acids 35 to 499 (465 residues), 444.7 bits, see alignment E=1.9e-137

Best Hits

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 71% identity to sal:Sala_2108)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (775 amino acids)

>GFF3425 DNA topoisomerase IV subunit A (EC 5.99.1.3) (Sphingobium sp. HT1-2)
MTDFRDPFDAIKDHPFDAALSQRYLVYALSTITARSLPDLRDGLKPVHRRLLWAMRLLRM
EPGGANPDVLVANPARNTTSYKKCARVVGDVIGKYHPHGDASVYDAMVRLAQDFSLRTPL
VDGQGNFGNIDGDNAAAMRYTEARLTQAAADLMAGLDEGTVDFRPTYNGEDEEPEVFPGL
FPNLLANGASGIAVGMATSIPPHNVGELLDAATLLIDSPEADDAALMEIVKGPDFPTGGV
LVDNQAIISEAYRTGRGSFRTRARFSTGRNEDGSWEAEGIEKLAGGTWQLVISEIPYQVQ
KSKLIEQIAALINDKKLPILEDVRDESDEAIRIVIVPKSRNVPVDILKDSLFRLTDLENR
FPLNLNVLDASHTPRVLGLRAVLVEWLKHQIEVLVRRAQHRLEKIAARLELLDGYIIAYL
NLDRVIEIIRTEDEPKDVMMDEFSLTDRQAEAILNMRLRSLRKLEEMELRREHAELVKEQ
AELEKLVESPARQRTRLKRDIADLRKRYGPDTVIGRRRTLVEEAGPAREIPLEAMIEREP
VTVILSERGWIRAMSGHRDLAAADTLKFKEGDGPQFAFHAYTTDKLLMATSSGRIYTLGA
DKLPGGRGFGDPVRMMVDMDDQGGIVALMPARTGGQLLLASSDGRGFLADVADILAETRK
GKQVVNVRTGAKLAVVHAVDPEADSIAVIGENRKLLVYSIIEMPKMARGQGVQMQRYRDG
GLSDAIAFRLSDGISWAMGGETGRTRTEADMTPWRVARGAAGRMPPTGFPRDNKF