Protein Info for GFF3421 in Variovorax sp. SCN45

Annotation: Acetoacetyl-CoA synthetase (EC 6.2.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 667 TIGR01217: acetoacetate-CoA ligase" amino acids 8 to 661 (654 residues), 689.3 bits, see alignment E=2.3e-211 PF16177: ACAS_N" amino acids 27 to 83 (57 residues), 35.6 bits, see alignment 9.9e-13 PF00501: AMP-binding" amino acids 92 to 482 (391 residues), 160.8 bits, see alignment E=6.9e-51

Best Hits

KEGG orthology group: K01907, acetoacetyl-CoA synthetase [EC: 6.2.1.16] (inferred from 90% identity to vpe:Varpa_0122)

Predicted SEED Role

"Acetoacetyl-CoA synthetase (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Polyhydroxybutyrate metabolism (EC 6.2.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.16

Use Curated BLAST to search for 6.2.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (667 amino acids)

>GFF3421 Acetoacetyl-CoA synthetase (EC 6.2.1.16) (Variovorax sp. SCN45)
MSAPNIPQIRLYQNWLREKHGLSFDSYDALWRWSTTELDAFWRSIWDYAAIQSPTPLASG
ATVLAERRMPGAQWFPGAQVNYAREVLRHVDAAHAAGMPAIVSDNERGDVREMSWPEMRR
QVASVALTLRALGVRRGDRVAAYMPNVPETMVAFLACSSIGAVWSVCAPDMGTAAVADRF
RQIEPKVLIAVDGVHYGGKPLDRSAVLQELRGQLPSVKKLVLVKSPFAANAVPHDIDWSR
AIGRYDDEVNAFEPEWLPFDHPIWIVYSSGTTGLPKPIVHGQGGIILTMHACGLHNDVGA
SYGANNLGERYHWYSSTGWVMWNSQLSGMAFGATICIYDGNPAGSKEKPDWSVLWRFVAR
HKVTFFGAGAAYFTNCMKAGLVARDCGDLSAVRALGSTGSPLPEEVQRWGSRQILDAGSS
EVWWCNISGGTDFCGAFVGGNRDLPEVPGQMQCRELGHAVEAWNDDGQPVIGEVGELVCT
QPIPSMPLYFWGDEGNARYLSSYFDTYPGVWRHGDWIRIGTDGGCIIYGRSDATINRQGL
RMGTSEIYSAVEGLPEVLDSMVVDLEYLGRDSYMPLFVVLRPGVALDDGMRARLNNAIKT
SLSPRFVPNDIFQVPEIPRTLSGKKQELPIKKLLLGQPLEKVVNREAMANPGSLEWYVAF
AAERASA