Protein Info for PS417_17460 in Pseudomonas simiae WCS417

Updated annotation (from data): Quinoprotein ethanol dehydrogenase QedA (EC 1.1.2.8)
Rationale: Important for ethanol utilization. Milder phenotypes on L-Arginine and L-Citrulline are not explained. 90% identical to A8R3S4 , which acts on longer primary alcohols as well
Original annotation: quinonprotein alcohol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 26 to 578 (553 residues), 782.8 bits, see alignment E=8.3e-240 PF13570: PQQ_3" amino acids 73 to 113 (41 residues), 19.1 bits, see alignment 2.1e-07 amino acids 114 to 162 (49 residues), 20.3 bits, see alignment 9.3e-08 amino acids 502 to 542 (41 residues), 26.5 bits, see alignment 1e-09 PF13360: PQQ_2" amino acids 88 to 233 (146 residues), 52.8 bits, see alignment E=6.9e-18 amino acids 492 to 560 (69 residues), 38.6 bits, see alignment E=1.4e-13 PF01011: PQQ" amino acids 95 to 121 (27 residues), 25.6 bits, see alignment (E = 1.2e-09) amino acids 144 to 169 (26 residues), 22.7 bits, see alignment (E = 9.1e-09) amino acids 524 to 560 (37 residues), 41.1 bits, see alignment 1.4e-14

Best Hits

Swiss-Prot: 91% identical to QEDH_PSEPU: Quinoprotein alcohol dehydrogenase (cytochrome c) (qedA) from Pseudomonas putida

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 95% identity to pfl:PFL_2216)

MetaCyc: 86% identical to alcohol dehydrogenase (cytochrome c550) monomer (Pseudomonas aeruginosa)
RXN-11333 [EC: 1.1.2.8]

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8, 1.1.99.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UN39 at UniProt or InterPro

Protein Sequence (615 amino acids)

>PS417_17460 Quinoprotein ethanol dehydrogenase QedA (EC 1.1.2.8) (Pseudomonas simiae WCS417)
MQAFVLVGSLSLSAVTFAAAEPAKPTRTVTWEDIANDHLTTQDVLQYGMGTNAQRWSPLA
QVNDKNVFKLTPAWSYSFGDEKQRGQESQAIVSDGVVYVTGSYSRVFALDAKTGKRLWTY
NHRLPDNIRPCCDVVNRGAAIFGDKIYFGTLDARLIALDKNTGKVVWNKKFGDHAAGYTL
TGAPVLIKDKVSGKVLLIHGSSGDEFGVVGQLFARDPDTGEEVWMRPFVEGHMGRLNGKD
STPTGDVKAPSWPDDPTTETGKVEAWSHGGGAPWQSASFDAETNTIIVGAGNPGPWNTWA
RTAKDGNPHDFDSLYTSGQVGVDPSTGEVKWFYQHTPNDAWDFSGNNELVLFDYKDKDGK
VVKATGHADRNGFFYVVDRNNGKLQNAFPFVDNITWASHIDLKTGRPVENEGQRPAKPLP
GETKGKPVEVSPPFLGGKNWNPMAYSQDTGLFYIPGNQWKEEYWTEEVNYKKGSAYLGMG
FRIKRMYDDHVGTLRAMNPTTGKVVWEHKEALPLWAGVLATKGNLVFTGTGDGFFKAFDA
KTGKELWKFQTGSGIVSPPITWEQDGEQYVGVTVGYGGAVPLWGGDMAELTKPVAQGGSF
WVFKIPSWDNKTAQK