Protein Info for GFF3403 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Alpha-methylacyl-CoA racemase (EC 5.1.99.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02515: CoA_transf_3
  • amino acids 4 to 338 (335 residues), 235.3 bits, E=6.1e-74

Best Hits

KEGG orthology group: None (inferred from 67% identity to rme:Rmet_2692)

Predicted SEED Role

"Alpha-methylacyl-CoA racemase (EC 5.1.99.4)" (EC 5.1.99.4)

MetaCyc Pathways

• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
• cholesterol degradation to androstenedione III (anaerobic) (1/22 steps found)
• bile acid biosynthesis, neutral pathway (2/29 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (9/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

• Bile acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 5.1.99.4

Use Curated BLAST to search for 5.1.99.4

Protein Sequence (347 amino acids)

>GFF3403 Alpha-methylacyl-CoA racemase (EC 5.1.99.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MQPLNHLHVIEFEGIGPGPLCGRVLADLGARVTVIARPGTSSVVDKLSGGGQPPLREGKT
VVELNLKHADDVAQALGLVASADALIEGNRPGVMERLGLGPAACAAVNPKLVYGRMTGWG
QQGPLAQAAGHDINYVALSGMLSLSAVHGQVPVSPPTVLGDAGGALGLALGIVSGVLQAR
QSGQGCVVDAAIVDVVAMLGGLAQWIAGTGGLGGPKPSVFHDSPFYDVYRCADGGFITLG
AIEPQFYALLVHKLGLTDVDPAAQFDTAAWPALKERLRTLFLAQPRQHWCDLLEGTDACF
APVLSLDEAARHPHNVVRGLYAPTPSGERRTGAAPRFTTLQGAHTHD