Protein Info for GFF3400 in Sphingobium sp. HT1-2

Annotation: L-lactate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01070: FMN_dh
  • amino acids 13 to 375 (363 residues), 433.8 bits, E=7.3e-134
• PF01645: Glu_synthase
  • amino acids 294 to 336 (43 residues), 20.8 bits, 3.1e-08

Best Hits

Swiss-Prot: 82% identical to LLDD_STRMK: L-lactate dehydrogenase (lldD) from Stenotrophomonas maltophilia (strain K279a)

KEGG orthology group: K00101, L-lactate dehydrogenase (cytochrome) [EC: 1.1.2.3] (inferred from 82% identity to sml:Smlt2908)

MetaCyc: 76% identical to L-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
1.1.5.M6 [EC: 1.1.5.M6]; RXN0-7227 [EC: 1.1.5.M6]

Predicted SEED Role

"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)

MetaCyc Pathways

• L-lactaldehyde degradation (aerobic) (1/2 steps found)
• methylglyoxal degradation IV (1/3 steps found)
• methylglyoxal degradation V (1/3 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)
• superpathway of methylglyoxal degradation (4/8 steps found)
• superpathway of fucose and rhamnose degradation (4/12 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.3

Use Curated BLAST to search for 1.1.2.3 or 1.1.5.M6

Protein Sequence (377 amino acids)

>GFF3400 L-lactate dehydrogenase (Sphingobium sp. HT1-2)
VIISAPTDYREAARRRLPPFLFHYIDGGANAEHTLRRNVTDLADVALRQRVLRDVADLSL
ETELFGSRLAMPVALAPVGLTGMYARRGEVQAARAAAAAGVPFTLSTVSVCAIDEVQRAS
SAPIWFQLYVLKDRGFMRDALERAQAAGVTTLVFTVDMPVPGSRYRDRHSGMSGPGAPLR
RVAQAITHPHWAWDVGLRGRPHDLGNISTYRGMPTKLEDYIGWLGNNFDPSIAWQDLQWI
RDFWKGSMVIKGILDPDDARDAVRFGADGIVVSNHGGRQLDGVLSSTRALPEIAEAVGGQ
IKVLADSGIRSGLDVVRMLALGADAVLLGRAFIYALAAAGEAGVANLLALFASEMRVAMA
LTGARSVGELDRGALVA