Protein Info for PS417_17360 in Pseudomonas simiae WCS417

Updated annotation (from data): D-galacturonate dehydrogenase (EC 1.1.1.203)
Rationale: Specifically important for: D-Galacturonic Acid monohydrate. KEGG now annotates it as uronate dehydrogenase which is correct (although less specific) (KEGG_correct)
Original annotation: NAD-dependent dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 13 to 158 (146 residues), 29.5 bits, E=1.2e-10
• PF01370: Epimerase
  • amino acids 15 to 177 (163 residues), 77.8 bits, E=2.7e-25
• PF01073: 3Beta_HSD
  • amino acids 16 to 155 (140 residues), 46.7 bits, E=6.6e-16
• PF13460: NAD_binding_10
  • amino acids 18 to 123 (106 residues), 30.7 bits, E=8.7e-11
• PF16363: GDP_Man_Dehyd
  • amino acids 50 to 172 (123 residues), 56.5 bits, E=1e-18
• PF07993: NAD_binding_4
  • amino acids 64 to 181 (118 residues), 26.8 bits, E=8.7e-10

Best Hits

Swiss-Prot: 83% identical to URODH_PSESM: Uronate dehydrogenase (udh) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: None (inferred from 96% identity to pfs:PFLU3949)

MetaCyc: 83% identical to uronic acid dehydrogenase subunit (Pseudomonas syringae)
Uronate dehydrogenase. [EC: 1.1.1.203]; 1.1.1.203 [EC: 1.1.1.203]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• D-glucuronate degradation III (2/2 steps found)
• D-glucuronate degradation II (4/5 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• colanic acid building blocks biosynthesis (8/11 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• D-galacturonate degradation III (1/2 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• D-galacturonate degradation II (3/5 steps found)
• D-galactose detoxification (1/3 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (2/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (13/31 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 1.1.1.203 or 5.1.3.2

Sequence Analysis Tools

See A0A1N7UN18 at UniProt or InterPro

Protein Sequence (274 amino acids)

>PS417_17360 D-galacturonate dehydrogenase (EC 1.1.1.203) (Pseudomonas simiae WCS417)
MTTTPTTPVPFNRLLLTGAAGGLGKVLRERLRPYAQVLRLSDIANMAPAADASEEVQPCD
LADKQAVHHLVEGVDAILHFGGVSVERSFEEVLGANISGIFHIYEAARRHGVKRVIFASS
NHVIGFYKQGEKLDAHSPRRPDSYYGLSKSYGEDMASFYFDRYGIETVSIRIGSSFPEPQ
NRRMMHTWLSFDDLTQLLERALYTPNVGHTVVYGMSDNLDTWWDNRYAAHLGFAPKDSSE
VFRAQVETQPPVSDNDPAKVYQGGAFCAAGPFGD