Protein Info for GFF3368 in Xanthobacter sp. DMC5

Annotation: L-lactate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 transmembrane" amino acids 324 to 340 (17 residues), see Phobius details PF01070: FMN_dh" amino acids 18 to 381 (364 residues), 449.3 bits, see alignment E=1.4e-138 PF00478: IMPDH" amino acids 240 to 337 (98 residues), 28.7 bits, see alignment E=1.1e-10 PF01645: Glu_synthase" amino acids 298 to 342 (45 residues), 21.1 bits, see alignment 2.4e-08

Best Hits

Swiss-Prot: 45% identical to LLDD_HAEDU: L-lactate dehydrogenase (lldD) from Haemophilus ducreyi (strain 35000HP / ATCC 700724)

KEGG orthology group: K00101, L-lactate dehydrogenase (cytochrome) [EC: 1.1.2.3] (inferred from 71% identity to met:M446_6648)

Predicted SEED Role

"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.3

Use Curated BLAST to search for 1.1.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (396 amino acids)

>GFF3368 L-lactate dehydrogenase (Xanthobacter sp. DMC5)
VIFPNAPITCVEDLRRLAARRVPRMFYDYVDCGSWTGATYRANEEDLAAIRLRQRVGIDI
SRRNLSSRMLGEDVAMPVALAPTGLAGMQWADGEILAARAAERFGVPFTLSTVSVCSIED
VAAQTSRPFWFQLYLMKDRAFMERLVARARDAGCSALVLTMDLPVQGQRHLDIKNGLSTP
PRPTLRNLVNLATKPRWCLNMARTERRTFRNIVGHVEGVSDASSLAHWVSEQFDQTLGWD
HVRWLRRIWDRKLIVKGIMDPEDARAAARAGADAIVVSNHGGRQLDGAPSSISALAAIAE
AVGARTEVLFDGGVRSGQDVLKALALGAAGVLIGRAFLYGLGAMGEAGVTRCLELIHREL
DLTMALCGLTDTSDAARALLNAPAYSEPAARVAAPV