Protein Info for GFF3360 in Xanthobacter sp. DMC5

Annotation: Acetophenone carboxylase delta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 593 PF02538: Hydantoinase_B" amino acids 19 to 542 (524 residues), 467.7 bits, see alignment E=2.3e-144

Best Hits

KEGG orthology group: K01474, N-methylhydantoinase B [EC: 3.5.2.14] (inferred from 49% identity to bxe:Bxe_C1059)

Predicted SEED Role

"N-methylhydantoinase B (EC 3.5.2.14)" in subsystem Hydantoin metabolism (EC 3.5.2.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.14

Use Curated BLAST to search for 3.5.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (593 amino acids)

>GFF3360 Acetophenone carboxylase delta subunit (Xanthobacter sp. DMC5)
VTTLATRTTGAMAARPPIDSITLEIIRHQLAAIPNQIERNIERTAFSPLVYEYKDYSVGF
VDPVGRLVVQSRGSLPIFVANALGTAVREGLEVLGPDHIHDGDIVVTNNPAWLGQHLNNV
VAYTPVRIEGELIGFFAVLVHWVDVGGSVPGSCVASNTTDVWQEGLQLPTLKLLERGRKR
PELFRLIAANTRFPAFLLGDVEAQIAGCQTGHDLVQEITARHGLEAVKAAIVAMWDGADH
AVKAALARARPGTYIARAELDDDGVTPGRRVPVDISVTLSAEEILVDLSGLGPQTAGPFN
AGRNGGAIAAARIACKYMFSPDTPVNDGDFRRLRVEIPDGRFLSASPDAAIGHSGSTVPT
VVDAILNAFGQALPERAAAAHHGIYGIHAFDGRNPETGERFQHLDTVTGGWGATAVADGP
SPYRSNGHGDVPDVPVEMQESFFPYRIEAKRLRPDSGGVGRHRGGLGVEKVYRILGDCRL
VVGFDRVACPPWGVHGGGAGLPASVEILRANGTRESFSKGERRLSEGDFAYVRSGGGGGF
GPAVERAVEDVLRDVRLGYVSREGAERDYGVVLSDDGIDEAATAARRKTMSSR