Protein Info for GFF3359 in Sphingobium sp. HT1-2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 PF00941: FAD_binding_5" amino acids 5 to 175 (171 residues), 145.7 bits, see alignment E=1.3e-46 PF03450: CO_deh_flav_C" amino acids 183 to 279 (97 residues), 75 bits, see alignment E=4.5e-25

Best Hits

Swiss-Prot: 39% identical to KDHA_PAENI: 6-hydroxypseudooxynicotine dehydrogenase complex subunit alpha (kdhA) from Paenarthrobacter nicotinovorans

KEGG orthology group: K03519, carbon-monoxide dehydrogenase medium subunit [EC: 1.2.99.2] (inferred from 46% identity to tro:trd_A0777)

MetaCyc: 39% identical to ketone dehydrogenase medium subunit (Paenarthrobacter nicotinovorans)
R162-RXN [EC: 1.5.99.14]

Predicted SEED Role

"Carbon monoxide dehydrogenase medium chain (EC 1.2.99.2)" in subsystem CO Dehydrogenase (EC 1.2.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.99.2

Use Curated BLAST to search for 1.2.99.2 or 1.5.99.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (289 amino acids)

>GFF3359 hypothetical protein (Sphingobium sp. HT1-2)
MKPAPFDYVRATSIDHGCALLAEAGGGAQLLAGGQTLMPLLALRMSQPFILIDIGHLPEL
QGIAAHAGGLRIGAATRQADALVHPLLAQALPVFVTALAHVGHHQTRNRGTIGGSIALGE
PAAEMPATAVALGASMTLRSVRGARTMAAADLYLGPYATAIAPDEMLVAIDYPAWPADAV
PLFREVARRPGDFALVGLVGALAIAEGRIERAGIAWFGMGPTPMRARQAEQALVGQAIDR
IDVQAIADLAIADSAPFDDGHASAAYRRSVGRRIFASSLAQALDRRLVA