Protein Info for GFF3351 in Sphingobium sp. HT1-2

Annotation: MFS family multidrug efflux protein, similarity to bicyclomycin resistance protein Bcr

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details amino acids 60 to 79 (20 residues), see Phobius details amino acids 88 to 107 (20 residues), see Phobius details amino acids 113 to 134 (22 residues), see Phobius details amino acids 146 to 170 (25 residues), see Phobius details amino acids 176 to 196 (21 residues), see Phobius details amino acids 228 to 249 (22 residues), see Phobius details amino acids 260 to 282 (23 residues), see Phobius details amino acids 294 to 312 (19 residues), see Phobius details amino acids 318 to 339 (22 residues), see Phobius details amino acids 351 to 373 (23 residues), see Phobius details amino acids 381 to 401 (21 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 20 to 394 (375 residues), 206.8 bits, see alignment E=3.5e-65 PF07690: MFS_1" amino acids 27 to 369 (343 residues), 117.9 bits, see alignment E=7.3e-38 PF06609: TRI12" amino acids 41 to 186 (146 residues), 38.6 bits, see alignment E=7.4e-14 PF00083: Sugar_tr" amino acids 60 to 199 (140 residues), 28.7 bits, see alignment E=1e-10

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 80% identity to sjp:SJA_C1-00730)

Predicted SEED Role

"MFS family multidrug efflux protein, similarity to bicyclomycin resistance protein Bcr"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (423 amino acids)

>GFF3351 MFS family multidrug efflux protein, similarity to bicyclomycin resistance protein Bcr (Sphingobium sp. HT1-2)
MAENSPMGAQPAAKDIHFREFVLLCACLMAMNALSIDPMLPALPDIGRDLHIANPNDRQL
IISFYFMGLGVGSLLFGVLSDQFGRKKVLGGAMILFILSSIACAGAHSFTMLLIARTSAG
FFAGASRVITVGIIRDRFKGDAMARVMSLIFAVFMLIPVMAPTFGQAILWIAPWRWIFWV
LAILATGILGWMAIRLPETLHPDNRIRINPRTILQTVGTIFRTRSSIGYMLASGVVMSGL
IGFILSVQQIFFDVFHAQKIFPLAFATIAGSMGIGSLVNSRLVSRFGARRLSQSALIAFI
LIAAVHLAVILAGQETIVTFMVLQAMTMMTVAFTASNFSAISMEPFSKGAGVASSFQAFL
TTAVSSALGSLVGRAFDGTTLPFTLGLLVFGSGSLLIVFWAERGKLFTRPHLNALREEYE
AMH