Protein Info for GFF3341 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Sigma factor RpoE negative regulatory protein RseB precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 signal peptide" amino acids 1 to 43 (43 residues), see Phobius details PF03888: MucB_RseB" amino acids 54 to 229 (176 residues), 190.8 bits, see alignment E=1.6e-60 PF17188: MucB_RseB_C" amino acids 248 to 344 (97 residues), 92.1 bits, see alignment E=2e-30

Best Hits

KEGG orthology group: K03598, sigma-E factor negative regulatory protein RseB (inferred from 56% identity to pol:Bpro_3641)

Predicted SEED Role

"Sigma factor RpoE negative regulatory protein RseB precursor" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (349 amino acids)

>GFF3341 Sigma factor RpoE negative regulatory protein RseB precursor (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTLPFRGDVSVLRFRHRRPVIRRWGVAALVALASGFMAPVHGQSAPPSNNVRSVNEWLTR
MHEASNRRSYIGTLVVSAGSAMSASKIWHVCDGSQQMERVDTLTGAPRTTIRRNKEVITF
VPEAKTAFVEKRDSLGMFPELLRTPNNLIPQFYSAREVGVQRVAGHLADMVEILPRDDLR
FGYRIWSERQTGLVVKLQTLGEQGAVMEQVAFSELQLDAPVSMEKLSKLMKDTRGYEVHK
PALKKTTAEAEGWRLKEPVPGFAAMSCHTREAPGGSEQTSMQWVFSDGLASVSLFVEPFD
AQRHTQEASATVGATNSVNRRVGEHWLTVMGEVPVATLRRFAVALERTR