Protein Info for GFF3336 in Sphingobium sp. HT1-2

Annotation: Aerobic cobaltochelatase CobT subunit (EC 6.6.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 608 PF06213: CobT" amino acids 5 to 276 (272 residues), 301.5 bits, see alignment E=6.5e-94 TIGR01651: cobaltochelatase, CobT subunit" amino acids 8 to 607 (600 residues), 860.4 bits, see alignment E=4.3e-263 PF11775: CobT_C" amino acids 391 to 606 (216 residues), 349 bits, see alignment E=1.2e-108

Best Hits

KEGG orthology group: K09883, cobaltochelatase CobT [EC: 6.6.1.2] (inferred from 87% identity to sch:Sphch_2362)

Predicted SEED Role

"Aerobic cobaltochelatase CobT subunit (EC 6.6.1.2)" in subsystem Coenzyme B12 biosynthesis or Conenzyme B12 related Hypothetical: Clusters with cobST (EC 6.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.6.1.2

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (608 amino acids)

>GFF3336 Aerobic cobaltochelatase CobT subunit (EC 6.6.1.2) (Sphingobium sp. HT1-2)
VTERSPLDAFKDVLAGTARAMTRDMEVEVSFTADTPHMVGKAIKVPTPSRALPADQVALA
RGFADANALKLRHHNARLHNAMAPGEAVARAVFDAVEQARVEAIGSRALEGVRANLNEAL
AMRLKSDPIRRARAADEVPLSTALALKVRERLTGQAVPEELQPAMKLVDQWIEDKAGSDL
DALGLAIDDQRAFQKLTQAMLEHLQLVDADVPPETDDPEPEDEGEDKEEEQEEGDSGQEE
SGDSDMNAEARSEDQGGETEEGDTDYSEEFDADAEDGADDMGEEGMMPVRPNRPTSDLPP
GFDYKSYTQKHDEVVTADDLCDEDELNRLRGFLDQQLVSLQGAVTKLANRLQRRLMAQQL
RSWDFDQEEGILDAARLARIVIDPTRSLTYKIERDTEFRDTVVTLLIDNSGSMRGRPISI
AAISADIMARTLERCGVKTEILGFTTRAWKGGQSREDWLAAGRPPMPGRLNDLRHIIYKK
ADEPWRRARRNLGLMMREGLLKENIDGEALLWAHARLIARPEERRILMVISDGAPVDDST
LSVNSGTYLERHLRQVIDWIENRSPVQLVAIGIGHDVTRYYRRAVTIMDAEQLGGTMVEQ
LAGLFDED