Protein Info for GFF3334 in Sphingobium sp. HT1-2

Annotation: Aerobic cobaltochelatase CobS subunit (EC 6.6.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 TIGR01650: cobaltochelatase, CobS subunit" amino acids 16 to 331 (316 residues), 587.9 bits, see alignment E=2.6e-181 PF12556: CobS_N" amino acids 18 to 48 (31 residues), 61.7 bits, see alignment (E = 4.2e-21) PF07728: AAA_5" amino acids 75 to 199 (125 residues), 42 bits, see alignment E=9.7e-15

Best Hits

Swiss-Prot: 71% identical to COBS_SINSX: Aerobic cobaltochelatase subunit CobS (cobS) from Sinorhizobium sp.

KEGG orthology group: K09882, cobaltochelatase CobS [EC: 6.6.1.2] (inferred from 93% identity to sch:Sphch_2359)

MetaCyc: 71% identical to CobS (Pseudomonas denitrificans (nom. rej.))

Predicted SEED Role

"Aerobic cobaltochelatase CobS subunit (EC 6.6.1.2)" in subsystem Coenzyme B12 biosynthesis or Conenzyme B12 related Hypothetical: Clusters with cobST (EC 6.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.6.1.2

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (335 amino acids)

>GFF3334 Aerobic cobaltochelatase CobS subunit (EC 6.6.1.2) (Sphingobium sp. HT1-2)
MTDISNIQPDARSETLMEAPDREVDVREMFGIDIDMKVPAFSQADERVPDLDPAYVFDPD
TTLAILAGFAFNRRVMVQGYHGTGKSSHIEQIAARLKWPCIRINLDAHISRIDLVGRDAI
VLKDGQQVTEFKEGLLPWSLQRPVALVFDEYDAGRPDVMFVIQRVLETDGKMTLLDQNRV
IRPNPHFRLFATANTVGLGDTTGLYHGTQQINQGQMDRWNLVVALNYLPAATEAQVVLAK
SGEYDHEGGRKEVESMIKVAELTRQGFINGDISTVMSPRTVISWAQNALIFKDVGFAFRL
SFLNKCDEAERPLVAEYYQRVFGKDLPESVASRAN