Protein Info for GFF3323 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: UDP-glucose 4-epimerase (EC 5.1.3.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01179: UDP-glucose 4-epimerase GalE
  • amino acids 2 to 340 (339 residues), 415.3 bits, E=7e-129
• PF01370: Epimerase
  • amino acids 3 to 266 (264 residues), 154.2 bits, E=8.4e-49
• PF02719: Polysacc_synt_2
  • amino acids 3 to 116 (114 residues), 24.7 bits, E=2.5e-09
• PF16363: GDP_Man_Dehyd
  • amino acids 4 to 329 (326 residues), 172.1 bits, E=4.6e-54
• PF01073: 3Beta_HSD
  • amino acids 5 to 256 (252 residues), 25.6 bits, E=1.2e-09

Best Hits

Swiss-Prot: 57% identical to GALE_YERPE: UDP-glucose 4-epimerase (galE) from Yersinia pestis

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 75% identity to pna:Pnap_1059)

MetaCyc: 53% identical to UDP-glucose 4-epimerase (Escherichia coli K-12 substr. MG1655)
UDP-glucose 4-epimerase. [EC: 5.1.3.2]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (10/11 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (5/6 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (3/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2

Protein Sequence (343 amino acids)

>GFF3323 UDP-glucose 4-epimerase (EC 5.1.3.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKVLLTGGAGYIGSHTAVALIEAGFEPVLLDNFANSHPVVLERLARITGQTMPLEQGDVL
DTPWLEAVLRRHRPVGVIHFAGDKAVGESVALPLKYFHNNIGGAVSLLRAMETVHAEDPS
LAPTLVFSSSATVYGDPATVPITEDFPRSHTNPYGHSKLVIEDMLTALCAARPAWRVGTL
RYFNPVGAHPSGLIGEDPAGIPNNLMPYVAQVAVGQREHLNVYGNDYPTPDGTGVRDYIH
VQDLAAGHVAAIQALLRRGESFTVNLGTGNGHSVLDVVRAFEKASGRPVPYKIAPRRAGD
VAQCWADPALALRLLGWQAKHTLDQMCADAWRWQSANPQGYRG