Protein Info for GFF3317 in Variovorax sp. SCN45

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 745 transmembrane" amino acids 93 to 113 (21 residues), see Phobius details amino acids 124 to 143 (20 residues), see Phobius details amino acids 159 to 177 (19 residues), see Phobius details amino acids 192 to 211 (20 residues), see Phobius details amino acids 346 to 368 (23 residues), see Phobius details amino acids 376 to 402 (27 residues), see Phobius details amino acids 698 to 716 (19 residues), see Phobius details amino acids 722 to 740 (19 residues), see Phobius details PF00403: HMA" amino acids 14 to 74 (61 residues), 57.1 bits, see alignment 3.9e-19 TIGR01511: copper-translocating P-type ATPase" amino acids 139 to 741 (603 residues), 554.5 bits, see alignment E=6.3e-170 TIGR01525: heavy metal translocating P-type ATPase" amino acids 157 to 740 (584 residues), 612.2 bits, see alignment E=2.3e-187 TIGR01494: HAD ATPase, P-type, family IC" amino acids 201 to 721 (521 residues), 273.5 bits, see alignment E=5.3e-85 PF00122: E1-E2_ATPase" amino acids 229 to 409 (181 residues), 202.4 bits, see alignment E=8.9e-64 PF00702: Hydrolase" amino acids 425 to 649 (225 residues), 119.3 bits, see alignment E=5.6e-38 PF12710: HAD" amino acids 525 to 645 (121 residues), 27.9 bits, see alignment E=6e-10

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 90% identity to vpe:Varpa_0033)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (745 amino acids)

>GFF3317 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Variovorax sp. SCN45)
MENLLQQPQTTLDLSVGGMTCASCVMRVERALKNVPGVQDVSVNLATESARIVAAPGDDI
DVRVRRAVRAAGYEPRAAASAADEAAALSPWHGFGPVAVGLLLSIPLMAPMVGSPFGQDW
MLPPWVQLLLAAPVQFWLGARFYRAGWHAAKARTGNMDLLVALGTSAAFGLSLWLWWRAA
TGEHAGHGEMPHLYFEASAVVITLVLLGKWLEARAKRQATSAIRALQQLRPEVAHLVGAR
GKESDVPLAEVMVGDHLVVRPGERVPADARVIEGQSEVDESMLTGEPLPVPKGPGDALTG
GAVNGDGRMVIEVSAVGAESVLARIIRLVEDAQAAKAPIQRLVDKVAAVFVPVVLVIAFV
TLAGWLIAGAGIETALIHAVAVLVIACPCALGLATPVAVMAGTGVAAKHGILIKDARALE
IAHRVDTVAFDKTGTLTLGRPVLTELLPVAGAIAAEDKLLAVAASLQGGSEHPLAKAVLA
AAAQRNLQAPALSAMQAMPGRGVRGNVDGANWAIASLRWCRELDAMPDATDVERLQAKGA
TVSALLRLDDAGSAHVQALLAFADEPKPQAAEAIRTLRARGLRVVMISGDNLRAAQAMAA
RLGIAAEDVRADVLPADKAAQVAALRKNGHVVAMVGDGANDAPALAAADVGIAMAPSGGG
TDVAMEAAGITLMRVDLALVAQAFELSGRTVAKIRQNLFWAFAYNVAGIPLAAFGLLSPV
VAGAAMALSSVSVMANALLLRRWKP