Protein Info for GFF3317 in Sphingobium sp. HT1-2

Annotation: Peptide chain release factor 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 525 TIGR00503: peptide chain release factor 3" amino acids 5 to 524 (520 residues), 631.8 bits, see alignment E=7.8e-194 PF00009: GTP_EFTU" amino acids 6 to 275 (270 residues), 178.2 bits, see alignment E=3.3e-56 PF01926: MMR_HSR1" amino acids 9 to 140 (132 residues), 27.4 bits, see alignment E=7.7e-10 TIGR00231: small GTP-binding protein domain" amino acids 10 to 147 (138 residues), 86 bits, see alignment E=2.5e-28 PF22042: EF-G_D2" amino acids 293 to 378 (86 residues), 75.1 bits, see alignment E=9.7e-25 PF03144: GTP_EFTU_D2" amino acids 311 to 376 (66 residues), 35 bits, see alignment E=3.8e-12 PF16658: RF3_C" amino acids 384 to 509 (126 residues), 150.3 bits, see alignment E=7e-48

Best Hits

Swiss-Prot: 53% identical to RF3_DESAA: Peptide chain release factor 3 (prfC) from Desulfatibacillum alkenivorans (strain AK-01)

KEGG orthology group: K02837, peptide chain release factor 3 (inferred from 90% identity to sjp:SJA_C1-34610)

Predicted SEED Role

"Peptide chain release factor 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (525 amino acids)

>GFF3317 Peptide chain release factor 3 (Sphingobium sp. HT1-2)
MSIPSRRTFAIISHPDAGKTTLTEKLLLEGGAIHLAGEVKARGANRRARSDWMKIEQQRG
ISVTSSVMTFERTRDGETITFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEPQT
RKLFEVCRLRNVPIITFVNKVDREGRELFGLLDEVADQLQLDVCPMSWPVGMGGEFEGIY
DFATNRLMQPTGPSKEFDGTRHQFSGLDDPKLADYLSERGLEKLREEAELAQGGYAEFDL
ERYRHGDLTPVYFGSALKLFGVTELIDALSAHAPPPRAQPAEPAPVEPEGSEVTGFIFKV
QANMDPMHRDRIAFMRLVSGKFRRGMKLTPSGSGKALAVHSPILFFAQDRELADEAFPGD
IIGIPNHGALRVGDTLSEKPGLRFTGLPNFAPEILRRVQLKDPTKTKQLRKALDDLSEEG
VIQVFYPEIGSNWIVGVVGQLQLEVLISRLEAEYKVAAVLEPSPFDTARWISGDDQAIKD
LVAYNGANMAKDRDGNLVFMARSAWDIGYQQERHPKVKFSATKER