Protein Info for GFF3313 in Sphingobium sp. HT1-2

Annotation: Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 804 (804 residues), 666 bits, see alignment E=4.1e-204 PF01588: tRNA_bind" amino acids 46 to 146 (101 residues), 74.6 bits, see alignment E=1.4e-24 PF03483: B3_4" amino acids 213 to 384 (172 residues), 205.5 bits, see alignment E=1.2e-64 PF03484: B5" amino acids 407 to 481 (75 residues), 44.5 bits, see alignment E=3.6e-15 PF17759: tRNA_synthFbeta" amino acids 499 to 692 (194 residues), 149.1 bits, see alignment E=3.3e-47 PF03147: FDX-ACB" amino acids 713 to 804 (92 residues), 76.7 bits, see alignment E=3.9e-25

Best Hits

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 86% identity to sch:Sphch_2341)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (805 amino acids)

>GFF3313 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) (Sphingobium sp. HT1-2)
MKFTLSWLKTHLKTDADLATILKTLNAIGLEVEDVENPAEKLGAFKIAKILTAERHPQAD
KLQVLSVDHGDGNPLQVVCGAPNARAGLIGVFGVEGAVVPVNGMVLKKTAIRGVESNGMM
CSTRELELGDDHDGIIELAGDAPVGEVYANWAGLNDPVIDVSITPNRQDCMGVRGIARDL
AAAGLGTLNAIIVPEIAGVGPGPDVRTDDVDGCPAFFARSVKGVTNGASPEWMARRLKAI
GQKPISALVDITNYIMIDLGRPLHVYDMATLKGGLVARKGKAGEEVLALNGKTYAIDETM
TVIADDAAVHDIGGIMGGEHSGAQDGTTDVLIECAYFTPERIAVTGQKLALTSDARGRFE
RGVDPAFLDDGLSIATSLVMTLCGGTASEATRAGTPPATPKTVAYAPDQCLALAGVDVPH
DVQKRILESLGFGVSGEVGGIEYQDGVPVSVPGHWSISVPTWRRDVDGWPDIVEEVVRIV
GLDHVPSTPLPRVPGVARPTATAEQLVERRVRRTAAARGLAEAINWSFISEKEAASVGGG
DWTLANPISEDLKVMRPSLLPGLLSATKRNVDRSATSIRLFELGRRYFKQGERATVGFVL
AGEKTARSWQSGKAQPFDAFDAKGEVIALLAAAGAPVGNLQSMGEASAAYHPGQSGTLRL
GPKVVLAEYGVLHPSLAKQFGLSGTVVAGEIFLDAIPAKRKSGFMRAPYAPPALQAVKRD
FAFLVPEAVEADALVRAVKGADKKAIVDARLFDVFTGPGVDEGHKSLAVEITLQPGEKSF
AQEELDAISAAVVKAAQKLGASLRG