Protein Info for GFF3312 in Sphingobium sp. HT1-2

Annotation: Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 363 PF02912: Phe_tRNA-synt_N" amino acids 20 to 87 (68 residues), 65.1 bits, see alignment E=4.5e-22 TIGR00468: phenylalanine--tRNA ligase, alpha subunit" amino acids 41 to 346 (306 residues), 325.2 bits, see alignment E=2.3e-101 PF01409: tRNA-synt_2d" amino acids 91 to 346 (256 residues), 327.3 bits, see alignment E=6.1e-102

Best Hits

Swiss-Prot: 79% identical to SYFA_SPHWW: Phenylalanine--tRNA ligase alpha subunit (pheS) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K01889, phenylalanyl-tRNA synthetase alpha chain [EC: 6.1.1.20] (inferred from 94% identity to sjp:SJA_C1-34560)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (363 amino acids)

>GFF3312 Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20) (Sphingobium sp. HT1-2)
MTEISALKAGLIADIEAADTLDALEGLRVGALGKNGVVTGLLKTLGGMSPEERLEKGPPI
QDLRESVTAAIADRKAALEQAALDARLASEKVDMTLPADLGPQGSVHAVSQVMDELAEIF
ADLGFSVATGPEIEDDWHNFTALNIPETHPARAMHDTFYFPDEEGQKKMLLRTHTSPVQI
RTMMTQEPPIRIIAPGRVYRSDSDATHTPMFHQIEGLVIDKGITLGHLKWTLETFLKAFF
EREDIVLRLRPSYFPFTEPSVEVDVGYTLTNGQRVIGGDGDAPGGGWLEVLGSGMVNRRV
IEACGLDPDEWQGFAFGTGVDRLAMLKYGMNDLRAFFDGDLRWLKHYGFSALDVPTLSGG
VGA