Protein Info for GFF3310 in Variovorax sp. SCN45

Annotation: Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 transmembrane" amino acids 29 to 54 (26 residues), see Phobius details PF00912: Transgly" amino acids 79 to 253 (175 residues), 221 bits, see alignment E=1.1e-69 TIGR02074: penicillin-binding protein, 1A family" amino acids 89 to 730 (642 residues), 606.9 bits, see alignment E=1.9e-186 PF17092: PCB_OB" amino acids 339 to 443 (105 residues), 89.4 bits, see alignment E=4.2e-29 PF00905: Transpeptidase" amino acids 447 to 693 (247 residues), 117.4 bits, see alignment E=1.1e-37

Best Hits

KEGG orthology group: None (inferred from 91% identity to vpe:Varpa_0026)

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (800 amino acids)

>GFF3310 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) (Variovorax sp. SCN45)
MSKRSSSTPPDDDGSAPADPNRRWKRIAGWGAVVVGSGALVVLVAAIVAVVAIYPKLPDV
SALSDYRPKLPLRVYSAEGQMIGEFGEERRNLTPFANIPKVMKDAVLAVEDARFYDHGGV
DYKGFLRAAVASLKGGRKQGASTITMQVARNVYLSSERTMSRKTYEILLALRLEQQLTKD
QILEIYLNQIYLGNRAYGFAAAAETYFGKPLQDVTIAEAAMLAGLPKAPGANNPVANPRR
ARARQLYVIDRMQETGFITAEQAAAAKKEELHLRDAADPNRLHAEYVAETVRQMMYAQYG
DSIYTSGLKVYTSLVAADQAAAYKSLRKGIMDYERRQAYRGPEKFVDLPTEQKEVDEAVD
DALAEHPDNGDVIAAVVLDANPKEISAVRANGETIQITGDGLKPAQSGLAAKAPPNIKIR
RGAVIRVAKTPKNTWEITQLPEVEAAFIGMDPRSGAIKALVGGFDFGKNKFNHVTQAWRQ
PGSSFKPFIYSAALEKGFTPATVVNDAPLYFDPSANGGQPWEPKNFEGTYEGPMPLRTAL
MKSKNLVTLRVLQSIGAPYAQDWVTKFGFDKDKQPANLPMGLGAGSVTPMQMAVGYSVFA
NGGYRVNPYLVTKVTDMKGKVLMETEPPVLDESRRAIPQRNAFIMSSLLQSVVRNGTGFK
AYQALKRDDLYGKTGTTNDSFDTWFAGFQPTMVGVAWVGYDTPRQLGVRGETGGSLSLPI
WTGYMQTALQGVPMTQPAEPPGVVRIDGELYFDDFTPGHNVASLGLDSTEAPQPVEELTS
APIGAPPPPEERNKILDFFR