Protein Info for PS417_16910 in Pseudomonas simiae WCS417

Annotation: multidrug resistance protein B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 509 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 52 to 72 (21 residues), see Phobius details amino acids 81 to 100 (20 residues), see Phobius details amino acids 106 to 131 (26 residues), see Phobius details amino acids 139 to 161 (23 residues), see Phobius details amino acids 167 to 188 (22 residues), see Phobius details amino acids 201 to 220 (20 residues), see Phobius details amino acids 232 to 251 (20 residues), see Phobius details amino acids 272 to 291 (20 residues), see Phobius details amino acids 303 to 323 (21 residues), see Phobius details amino acids 334 to 354 (21 residues), see Phobius details amino acids 360 to 385 (26 residues), see Phobius details amino acids 398 to 421 (24 residues), see Phobius details amino acids 476 to 494 (19 residues), see Phobius details PF06609: TRI12" amino acids 8 to 280 (273 residues), 28.9 bits, see alignment E=6.4e-11 TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 13 to 493 (481 residues), 512.2 bits, see alignment E=7e-158 PF07690: MFS_1" amino acids 19 to 413 (395 residues), 186.2 bits, see alignment E=1.3e-58 PF00083: Sugar_tr" amino acids 23 to 184 (162 residues), 36.4 bits, see alignment E=4.5e-13

Best Hits

Swiss-Prot: 51% identical to EMRB_HAEIN: Multidrug export protein EmrB (emrB) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 99% identity to pfs:PFLU3875)

MetaCyc: 49% identical to tripartite efflux pump membrane subunit EmrY (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U0S6 at UniProt or InterPro

Protein Sequence (509 amino acids)

>PS417_16910 multidrug resistance protein B (Pseudomonas simiae WCS417)
MSNNASFTPPSLLMATIGLSLATFMQVLDTTIANVALPTISGNLGVSSEQGTWVITSFAV
SNAIALPLTGWLSRRFGEVKLFLWATLLFVLASFLCGISTSMPELIAFRVIQGLVAGPLY
PMTQTLLIAVYPPARRGMALALLAMVTVVAPIAGPILGGWITDSYSWPWIFFINVPIGIF
AVMVVRSQLKKRPVETSYQPMDYVGLLSLIVGVGALQIILDKGNDLDWFESNFIIIGAAI
SVIALAVFVIWEMTDKHPVVNLRLFAYRNFRIGTIVLILGYAGFFGINLILPQWLQTQMG
YTATWAGLAVAPIGILPVLMSPFVGKYAHKFDLRLLAGLAFLAIGLSCFMRAGFTNEVDF
QHIALVQLFMGIGVALFFMPTLSILMSDLPPHQIADGAGLATFLRTLGGSFAASLTTWIW
IRRADQHHAYMSENMTTYDSATRDALQALGGAGQKAYTQLDQILTSQAYMMSTVDYFTLL
GWMFMGLMLLVWLAKPPFGAKAGPEASGH