Protein Info for PS417_16895 in Pseudomonas simiae WCS417

Annotation: glutamate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 PF00749: tRNA-synt_1c" amino acids 38 to 332 (295 residues), 356.7 bits, see alignment E=1.3e-110 TIGR00440: glutamine--tRNA ligase" amino acids 40 to 564 (525 residues), 790.3 bits, see alignment E=4.9e-242 PF03950: tRNA-synt_1c_C" amino acids 351 to 451 (101 residues), 107.5 bits, see alignment E=4.8e-35 PF20974: tRNA-synt_1c_C2" amino acids 467 to 543 (77 residues), 91.7 bits, see alignment E=5.8e-30

Best Hits

Swiss-Prot: 90% identical to SYQ_PSESM: Glutamine--tRNA ligase (glnS) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 98% identity to pfs:PFLU3872)

MetaCyc: 63% identical to glutamine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glutamine--tRNA ligase. [EC: 6.1.1.18]

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U598 at UniProt or InterPro

Protein Sequence (566 amino acids)

>PS417_16895 glutamate--tRNA ligase (Pseudomonas simiae WCS417)
MSKPTVDPTSNSKAGPAVPVNFLRPIIQADLDSGKHTQIVTRFPPEPNGYLHIGHAKSIC
VNFGLAQEFGGVTHLRFDDTNPAKEDQEYIDAIESDIKWLGFEWSGEVRYASKYFDQLFD
WAVELIKAGKAYVDDLTPEQAKEYRGTLTEPGKNSPFRDRSVEENLDWFARMRAGEFPDG
ARVLRAKIDMASPNMNLRDPIMYRIRHAHHHQTGDKWCIYPNYDFTHGQSDAIEGITHSI
CTLEFESHRPLYEWFLDSLPVPAHPRQYEFSRLNLNYTITSKRKLKQLVDEKHVHGWDDP
RMSTLSGFRRRGYTPASIRNFCDMVGTNRSDGVVDFGMLEFSIRQDLDANAPRAMCVLRP
LKVVITNYPEDKVDHLELPRHPQKEELGVRKLPFAREIYIDRDDFMEEPPKGYKRLEPNG
EVRLRGSYVIRADEAIKDADGNIVELRCSYDPDTLGKNPEGRKVKGVIHWVPAAASIECE
VRLYDRLFRSPNPEKAEDSASFLDNINPDSLQVLTGCRAEPSLADAQPEDRFQFEREGYF
CADIKDSKPGQPVFNRTVTLRDSWGQ