Protein Info for GFF330 in Sphingobium sp. HT1-2

Annotation: Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 PF01739: CheR" amino acids 78 to 262 (185 residues), 158.6 bits, see alignment E=6.6e-51

Best Hits

Swiss-Prot: 32% identical to CHER_VIBPA: Chemotaxis protein methyltransferase (cheR) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K00575, chemotaxis protein methyltransferase CheR [EC: 2.1.1.80] (inferred from 76% identity to sjp:SJA_C1-10470)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80

Use Curated BLAST to search for 2.1.1.80

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (289 amino acids)

>GFF330 Chemotaxis protein methyltransferase CheR (EC 2.1.1.80) (Sphingobium sp. HT1-2)
MALPPSRMGSGAARILTGLLEARTGQVLSEGRAWRMETALKPVLRDHGLQDIDELAGKLL
RSRNPRLDEDVVNALLNNESSFFRDLQIFDLIHRQILPRIQEARGDRVLRIWSAGCSTGQ
EAYSLAIRFCNEAARWAGWRIEILATDISSAAIDQARSGIFSQMDVQRGLPIGDLIKWFE
PAGDDWRANPELRRMIDFRQDNLFAARVPSGAYDLLLCRNVLLYFSPERRQSVFDLLARH
SHDGSVLLLGAGETVIGQSEEFVPHPEFRGSYARRGYSADAGNSARRAG