Protein Info for GFF33 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR02432: tRNA(Ile)-lysidine synthetase" amino acids 15 to 197 (183 residues), 164.9 bits, see alignment E=2e-52 PF01171: ATP_bind_3" amino acids 16 to 192 (177 residues), 198.7 bits, see alignment E=1.1e-62 PF09179: TilS" amino acids 242 to 309 (68 residues), 75.7 bits, see alignment E=4.3e-25 PF11734: TilS_C" amino acids 357 to 428 (72 residues), 90.6 bits, see alignment E=4.9e-30 TIGR02433: tRNA(Ile)-lysidine synthetase, C-terminal domain" amino acids 357 to 402 (46 residues), 59.1 bits, see alignment 2.2e-20

Best Hits

Swiss-Prot: 100% identical to TILS_SALTY: tRNA(Ile)-lysidine synthase (tilS) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K04075, tRNA(Ile)-lysidine synthase [EC: 6.3.4.-] (inferred from 99% identity to seh:SeHA_C0274)

MetaCyc: 76% identical to tRNAIle-lysidine synthetase (Escherichia coli K-12 substr. MG1655)
RXN-1961 [EC: 6.3.4.19]

Predicted SEED Role

"tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)" (EC 6.3.4.19)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.- or 6.3.4.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (430 amino acids)

>GFF33 tRNA(Ile)-lysidine synthetase (EC 6.3.4.19) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MTTLTLNTSLLSSRRILAAFSGGLDSTVLLHQLVLWRERHPDVTLRAIHIHHGLSPHADS
WVRHCETVCERWQVPLVVERVTLADNGLGIEAHAREARYRAFAQTLLPGEVLATAQHLDD
QCETFLLALKRGSGPAGLSAMGERSPFAGTLLLRPLLRETRKTLEQWAVRHGLCWIEDES
NQDDAYDRNFLRLRALPLLQQRWPHFPAAVARSATLCAEQERLLDELLASDLTDCITAEG
TLRLSPLMSMSDVRRAAILRRWLAMRNAPMPSRDALERIWQEVALARDDASPCLRFGDHE
IRRYQSQLWWIKSVAGQHETTVAWPVWQTPLALPAGLGTVQLVPGGELRRPREEESVSIR
FKAPGVLHIVGRNGGRKLKKIWQEQGIPPWRRDTTPLLFYGETLIAAAGVFVTREGAAED
KEGVSLVWHA