Protein Info for GFF3298 in Sphingobium sp. HT1-2

Annotation: Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00749: tRNA-synt_1c
  • amino acids 3 to 306 (304 residues), 241.1 bits, E=1.5e-75
• TIGR00464: glutamate--tRNA ligase
  • amino acids 3 to 328 (326 residues), 318.6 bits, E=3.8e-99
• PF19269: Anticodon_2
  • amino acids 366 to 442 (77 residues), 46.4 bits, E=5.6e-16

Best Hits

Swiss-Prot: 68% identical to SYE1_SPHWW: Glutamate--tRNA ligase 1 (gltX1) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 89% identity to sch:Sphch_2331)

Predicted SEED Role

"Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.24)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
• tRNA charging (19/21 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• superpathway of heme b biosynthesis from glutamate (7/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (4/6 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (25/36 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.17

Use Curated BLAST to search for 6.1.1.17 or 6.1.1.24

Protein Sequence (444 amino acids)

>GFF3298 Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24) (Sphingobium sp. HT1-2)
MTVITRFAPSPTGHLHVGNIRAALHNWLWARKSGGQFLLRLDDTDLERSRPEYADSIKAD
LRWLGLDWDGEEKQSERFALYEEKFEALKASGHVYPAYETQQELELRRKVLLGRGLPPVY
DRAALALTPDQIAAYEAEGRTPHWRFKLDHEQPIVWTDLIRGEQRFDPKLLSDPVIRRAD
GSWLYMLPSVIDDVDMGVTHVLRGEDHVSNTATQIQMFSALGASLPAFAHEALLTGSEGK
LSKRLGSLGVAHFREIGLEPAAIASLLARLGSSQPIEPFADRAPLIETFDFAHFGRAPAR
FDEAELASLNQKIVHQLPYDAVADRLPAGMDAAAWAAIRPNLETVAGAADWWQIVTGPID
GPEVADEDRDFLAAAHRILSAVPFDADIWRTLTGALKEETGRKGKTLFQPLRRALTGLDH
GPDMGQLLPLIGREEALKRLAVAG