Protein Info for GFF3296 in Xanthobacter sp. DMC5

Annotation: Glycine--tRNA ligase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR00388: glycine--tRNA ligase, alpha subunit" amino acids 15 to 301 (287 residues), 459.6 bits, see alignment E=2e-142 PF02091: tRNA-synt_2e" amino acids 16 to 303 (288 residues), 473.7 bits, see alignment E=8.7e-147

Best Hits

Swiss-Prot: 84% identical to SYGA_BRADU: Glycine--tRNA ligase alpha subunit (glyQ) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01878, glycyl-tRNA synthetase alpha chain [EC: 6.1.1.14] (inferred from 94% identity to xau:Xaut_3330)

MetaCyc: 65% identical to glycine--tRNA ligase subunit alpha (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (310 amino acids)

>GFF3296 Glycine--tRNA ligase alpha subunit (Xanthobacter sp. DMC5)
MIDQSLPAHMRPDRSFQALLLTLQRFWADQGCVVLQPYDMEVGAGTFHPATTLRALGPKH
WKAAYVQPSRRPKDGRYGENPNRLQHYYQFQVILKPSPENLQELYLQSLAAIGVDLKLHD
VRFVEDDWESPTLGAWGLGWECWCDGMEVSQFTYFQQVAGFECAPVSGELTYGLERLAMY
VQGVENVYDLNFNGREGAEKTTYGDVFLQAEQEYSRHNFEAADTTMLFEQFRMAEAACHK
YLAFGDKGETHEMALPAYDQCIKASHVFNLLDARGVISVTERQSYILRVRELAKACGAAW
LKTAGGGAAA