Protein Info for GFF3293 in Xanthobacter sp. DMC5

Annotation: Glycine--tRNA ligase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 732 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 3 to 507 (505 residues), 478 bits, see alignment E=3.7e-147 PF02092: tRNA_synt_2f" amino acids 4 to 587 (584 residues), 627.9 bits, see alignment E=1.9e-192 PF05746: DALR_1" amino acids 619 to 718 (100 residues), 35.3 bits, see alignment E=1.1e-12

Best Hits

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (732 amino acids)

>GFF3293 Glycine--tRNA ligase beta subunit (Xanthobacter sp. DMC5)
MPDLLLELFSEEIPARMQAQAADALKKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLP
GRQADQKEEKKGPRVGAPEQAVAGFLKAAGLASLDQATIQSDPKKGDFYVAVVEKPGRAT
TEVVAEIVPDIIRKFPWPKSMRWGSGTLRWVRPLHSIVATFGAEGDEPEVVHFSVDGIEA
SNVTRGHRFMAPEPFTVKRLDDWMAKLEAAKVVADRDRRKEIILNDAKDLALAAGLELVE
DEGLLDEVCGLVEWPVVLMGSFEARFLDIPDEVIRATIRANQKCFVLKDPKTGRLANRFI
LISNLVASDGGKAIVAGNERVIRARLSDAKFFWDTDRLNGLASRMPKFQSITFHEKLGSQ
AERIERIVRLAKELAPKVGAPVALAEEAALVAKADLLTEVVGEFPELQGLMGRYYAQGEN
LPPEVAAACEEHYKPQGPNDRVPTAPVSVAVALADKIDTLVGFWGIRELPSGSKDPYALR
RAALGIVRIILEGGLRIKLKSEIYNEVHRRLYLVRQASDHVYLKFGSLVRAEMYGGLDEE
KIRDFETELKDVHSKDISGDVLTFLADRLKVYLRDRGARHDLVDAVFALEGQDDLLLVVR
RVEALAGFLATEDGKNLLAGYRRAVNILRIEEKKDGRAFAGPTVAALLKEPAEEHLAAAI
DDVKARVSKAVAAEDFEAGMTALSDLRQPVDAFFDAVTVNDADPALRENRLKLLDEIRAA
TRGIADFDRIEG