Protein Info for GFF3292 in Sphingobium sp. HT1-2

Annotation: N(6)-L-threonylcarbamoyladenine synthase (EC 2.3.1.234)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 348 TIGR03723: tRNA threonylcarbamoyl adenosine modification protein TsaD" amino acids 8 to 316 (309 residues), 415.5 bits, see alignment E=1.3e-128 TIGR00329: metallohydrolase, glycoprotease/Kae1 family" amino acids 9 to 309 (301 residues), 326.2 bits, see alignment E=2e-101 PF00814: TsaD" amino acids 28 to 310 (283 residues), 314.5 bits, see alignment E=3.7e-98

Best Hits

Swiss-Prot: 80% identical to TSAD_SPHAL: tRNA N6-adenosine threonylcarbamoyltransferase (tsaD) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K01409, O-sialoglycoprotein endopeptidase [EC: 3.4.24.57] (inferred from 88% identity to sjp:SJA_C1-03150)

Predicted SEED Role

"TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (348 amino acids)

>GFF3292 N(6)-L-threonylcarbamoyladenine synthase (EC 2.3.1.234) (Sphingobium sp. HT1-2)
MEAAMTIILGLESSCDETAAALVTADGQILAHRLATQEDAHRPYGGVVPEIAARAHVEAL
APLVEAALADAKMTLADVDVIAATAGPGLIGGVMVGLVTGKALAHASGKPLVAVNHLEGH
ALSPRLADQNLQFPYLLLLVSGGHCQLLRVEGVGQYRRLATTIDDAAGEAFDKTAKLLGL
GFPGGPAVEKAAAKGNPRAVPLPRPLVGTDEPHFSFAGLKSAVMRAAQSGQYAVEDIAAS
FQQAVIDCLLDRTRRALAASEGVTALVVAGGVAANQSVRGALTALAQDHDLPFVAPPLWL
CTDNAAMIAWAGAERYAAGLTDDLTIPARPRWPLDPTAEKARGAGVKA