Protein Info for GFF3280 in Xanthobacter sp. DMC5

Annotation: Hydrogenobyrinate a,c-diamide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 TIGR00379: cobyrinic acid a,c-diamide synthase" amino acids 9 to 411 (403 residues), 315.7 bits, see alignment E=2.6e-98 PF01656: CbiA" amino acids 9 to 193 (185 residues), 65.8 bits, see alignment E=5.8e-22 PF13500: AAA_26" amino acids 97 to 187 (91 residues), 34.1 bits, see alignment E=3.8e-12 PF07685: GATase_3" amino acids 253 to 442 (190 residues), 110.4 bits, see alignment E=1.3e-35

Best Hits

Swiss-Prot: 64% identical to COBB_RHILO: Hydrogenobyrinate a,c-diamide synthase (cobB) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K02224, cobyrinic acid a,c-diamide synthase [EC: 6.3.1.- 6.3.5.9] (inferred from 81% identity to xau:Xaut_3276)

Predicted SEED Role

"Cobyrinic acid A,C-diamide synthase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.- or 6.3.5.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (444 amino acids)

>GFF3280 Hydrogenobyrinate a,c-diamide synthase (Xanthobacter sp. DMC5)
MSDAPRGLLIAAPRSGSGKTTVTLALLAALRRRGKAVRAIKSGPDYIDPAFHAAATGRPG
LNLDSWAMPPALLDHLAGLAGEGAEIVIGEGAMGLFDGVAVEKGRTGAGADVAARLGLPV
LLVLDVSGQSQTAAAVLRGFIGHDPAVRIAGVVLNKLGSERHERMIREAVAPLGVPVLGT
VPRAADIVLPERHLGLVQAGETDDLPARLAALADLAEAHLDLDGILAAAAPLQAGAGEGQ
GHNTALLPPPGQRIALAQDAAFGFVYAHMLAGWRGQGAEIVPFSPLADEAPDASCDALWL
PGGYPELHAGPLAAAERFRAGVHAFAAAGKPVHGECGGYMALGAGLVDAEGVRHAMLGLL
ELETSFAKRRMNLGYRQARLMAPSPLGPEGSFVRGHEFHYATVLSRGADQPLADLSDAEG
RPLGPAGSRRGSVTGSFFHAIARG