Protein Info for GFF3272 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Putative two component system histidine kinase YedV

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 157 to 176 (20 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 2 to 444 (443 residues), 328.8 bits, see alignment E=3e-102 PF00672: HAMP" amino acids 174 to 226 (53 residues), 30.1 bits, see alignment 1e-10 PF00512: HisKA" amino acids 232 to 296 (65 residues), 44.1 bits, see alignment E=3.5e-15 PF02518: HATPase_c" amino acids 341 to 445 (105 residues), 67.5 bits, see alignment E=2.8e-22 PF14501: HATPase_c_5" amino acids 345 to 443 (99 residues), 22.7 bits, see alignment E=1.5e-08

Best Hits

Swiss-Prot: 55% identical to HPRS_ECOLI: Sensor histidine kinase HprS (hprS) from Escherichia coli (strain K12)

KEGG orthology group: K02484, two-component system, OmpR family, sensor kinase [EC: 2.7.13.3] (inferred from 100% identity to stm:STM1095)

MetaCyc: 55% identical to sensor histidine kinase HprS (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Putative two component system histidine kinase YedV" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (449 amino acids)

>GFF3272 Putative two component system histidine kinase YedV (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MTLRLTISFIAILILACTGISWTLYNALSKELTYRDDMTLINRAAQMQQLLLDGARPENL
PLYFNRMVDTKQDILLIHSATGHNVAINHSGIPDQRFNEIPLTKNITRETLFRQAVQGTE
LTAVRVSARSGDDPLTLTIARLATERRQMLAQYRRNSLLMSLIAILVCSALSPLVIRNGL
RAITSLSRLTAATDSGTLRQPLAEQALPVELRPLGQALNTMRQKLSDDFERLNQFADDLA
HELRTPVNILLGKNQVMLSQERSAEEYQQALVDNIEELEGLSRLTENILFLARAEHQNIA
VKKQPVSLNALVENMLDYLSPLAEEKRICFINQCQGTVWADEILLQRVLSNLLTNAIRYS
DENAVIRIESAYDDNVAEIRVANPGSHPADADKLFRRFWRGDNARHTAGFGLGLSLVNAI
ALLHGGSASYRYADEHNIFSVRLPDSGDS