Protein Info for PS417_01660 in Pseudomonas simiae WCS417

Annotation: tRNA s(4)U8 sulfurtransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 TIGR00342: tRNA sulfurtransferase ThiI" amino acids 4 to 371 (368 residues), 327.8 bits, see alignment E=9.1e-102 PF22025: ThiI_fer" amino acids 9 to 78 (70 residues), 29.1 bits, see alignment E=2.7e-10 PF02926: THUMP" amino acids 88 to 163 (76 residues), 45.1 bits, see alignment E=2.9e-15 PF02568: ThiI" amino acids 179 to 370 (192 residues), 204 bits, see alignment E=4.8e-64 TIGR04271: thiazole biosynthesis domain" amino acids 385 to 483 (99 residues), 119.6 bits, see alignment E=5.3e-39 PF00581: Rhodanese" amino acids 411 to 482 (72 residues), 22.5 bits, see alignment E=3.2e-08

Best Hits

Swiss-Prot: 96% identical to THII_PSEFS: tRNA sulfurtransferase (thiI) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K03151, thiamine biosynthesis protein ThiI (inferred from 96% identity to pfs:PFLU0349)

MetaCyc: 50% identical to tRNA uridine 4-sulfurtransferase (Escherichia coli K-12 substr. MG1655)
tRNA sulfurtransferase. [EC: 2.8.1.4]; 2.8.1.- [EC: 2.8.1.4]

Predicted SEED Role

"Thiamine biosynthesis protein thiI"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UEM1 at UniProt or InterPro

Protein Sequence (484 amino acids)

>PS417_01660 tRNA s(4)U8 sulfurtransferase (Pseudomonas simiae WCS417)
MKLIVKVFPEITIKSRPVRTRFIRQLAKNIRAVLRDLDPAVVVNGVWDNLELETRVTEPK
ALKDMTERLSCMPGIAHFLQVDEYPLGDFDDITEKCKQHYGSELEGKIFSVRCKRAGKHT
FSSMDVEKYVGSKLRRECGASGISLKAPQIEVRMEIRDQRLFVIHSQHNSIGGYPLGALE
QTLVLMSGGFDSTVAAYQIMRRGLMSHFCFFNLGGRAHELGVMEVAHFIWKKYGSSQRVL
FVSVPFEEVLGEILGKVDNGHMGVVLKRMMLRAASRIADKLQIDALVTGEAISQVSSQTL
PNLSIIDCVTEKLVLRPLIASHKQDIIDLAEQIGTADFAKHMPEYCGVISVNPKTHAKRH
RVEHEEKEFDMAILERALENAKLVPIDRVIDELGQDIKIEEVSEALAGQIVIDIRHPDAA
EDEPLEIAGIDVQTLPFYALNARFKELDNSRQYLLYCDKGVMSRLHAHHLLSEGHANVRV
YRPS