Protein Info for GFF3257 in Sphingobium sp. HT1-2

Annotation: 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase
  • amino acids 69 to 261 (193 residues), 236.2 bits, E=1.3e-74
• PF00926: DHBP_synthase
  • amino acids 69 to 260 (192 residues), 279.6 bits, E=1.1e-87
• PF00925: GTP_cyclohydro2
  • amino acids 270 to 423 (154 residues), 116.4 bits, E=1e-37

Best Hits

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 85% identity to sch:Sphch_2369)

Predicted SEED Role

No annotation

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
• toxoflavin biosynthesis (3/7 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25, 4.1.99.12

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Protein Sequence (426 amino acids)

>GFF3257 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25) (Sphingobium sp. HT1-2)
MSSSLIDSVRALVTDGGMSRSGLARAAGLHANSLRKLGEGDWNPTAETLGKLEAYLLKRE
GGTALASPEEIINEARNGRMFILVDDEDRENEGDLVIPAQMATPDAINFMATHGRGLICL
ALTKDRVDQLGLDLMSRNNGTRHETAFTVSIEAREGVDTGISAADRARTVSVAIDASKTR
ADIVTPGHVFPLIARDGGVLVRTGHTEAAVDVARLAGLNPSGVICEVMKDDGTMARLDDL
IIFAQKHKMKIGTIRDLIAYRRRHDHVVERRAETSFESKWGGEWKAITFFNRATQSEQLV
LQKGRISPDQPTLVRMHQLAPLSDIFGAEGPRGDMLARSMEIIDKEGAGVVVLLRDGEPD
MLTRMIQAHAGKQPGGMDELRDYGVGAQILAELGVHDMILLSNTQHSLIALDGYDLAVVG
QRPIEL