Protein Info for GFF3249 in Sphingobium sp. HT1-2

Annotation: CzcABC family efflux RND transporter, membrane fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 transmembrane" amino acids 28 to 48 (21 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 65 to 384 (320 residues), 211.5 bits, see alignment E=7.5e-67 PF16576: HlyD_D23" amino acids 99 to 304 (206 residues), 74.6 bits, see alignment E=1.4e-24 PF13533: Biotin_lipoyl_2" amino acids 100 to 138 (39 residues), 24.2 bits, see alignment 4.5e-09 PF13437: HlyD_3" amino acids 202 to 301 (100 residues), 49.1 bits, see alignment E=1.7e-16

Best Hits

KEGG orthology group: None (inferred from 74% identity to sch:Sphch_2376)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (390 amino acids)

>GFF3249 CzcABC family efflux RND transporter, membrane fusion protein (Sphingobium sp. HT1-2)
MNYETTLTGESSAILIDDEDRIRRRRRLLIIGGVVAVALVAAAAALSMRGKDAAAPDAAP
QAPVVSVLTPGQTMVARTVSSTGSLAARVDMPVGVVGEGGMVTRVLVQPGDWVRAGQVLA
TVERSVQSEQVNSLAAQVEVQRANAKLAQAQLERAKALVGRGFISAADIDQRTATRDQAV
AQVRVAEAQLAEQRARTGRLDIRAPASGLVLTRTVEPGQIVQAGGGTLFRMAKDGEMEMQ
AQVAEADLATLRAGNSATVTPVGSNQHFSGRVWQVSPVVDPQTRQGIARIAVAYNDAIRP
GGFASATITSGSGNTPLLPESAVQSDAKGSYVYIVNGKNQVERRDVKVGQVSDTGVAITS
GITGAERIVTSAGAFLTVGQKVKPEALKAR