Protein Info for GFF3239 in Sphingobium sp. HT1-2

Annotation: Thiamine-monophosphate kinase (EC 2.7.4.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01379: thiamine-phosphate kinase
  • amino acids 13 to 304 (292 residues), 244.8 bits, E=5.5e-77
• PF00586: AIRS
  • amino acids 25 to 135 (111 residues), 85.2 bits, E=4.4e-28
• PF02769: AIRS_C
  • amino acids 148 to 230 (83 residues), 27.8 bits, E=2.6e-10

Best Hits

KEGG orthology group: K00946, thiamine-monophosphate kinase [EC: 2.7.4.16] (inferred from 83% identity to sch:Sphch_2385)

Predicted SEED Role

"Thiamine-monophosphate kinase (EC 2.7.4.16)" in subsystem Thiamin biosynthesis (EC 2.7.4.16)

MetaCyc Pathways

• thiamine diphosphate biosynthesis I (E. coli) (2/2 steps found)
• thiamine diphosphate biosynthesis II (Bacillus) (2/2 steps found)
• thiamine diphosphate salvage II (4/5 steps found)
• thiamine diphosphate salvage V (2/3 steps found)
• thiamine diphosphate salvage I (1/2 steps found)
• superpathway of thiamine diphosphate biosynthesis II (7/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (6/10 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.16

Protein Sequence (305 amino acids)

>GFF3239 Thiamine-monophosphate kinase (EC 2.7.4.16) (Sphingobium sp. HT1-2)
MSAESRFLTLLRLLAHDPAAQGLQDDVAVLPVGATRLILTSDTMVEGVHYLPTDPPADIG
WKLAAVNLSDLAAKGAKPVGCLLNYSLSGDDGWDAAFLEGLGEALERYAMPLLGGDTVSM
PAGAPRSYSLTAIGEATSTPVPSRAGAQAGDRLYVTGPVGDAGIGLDLARADPAASGPLV
AAYRRPRPRLAEGALLARLATAMMDLSDGLLIDAARMGAASNLAVTIDHIPLSPALESVR
GASTAIQLAAARAGDDYELLFALPPGVSPPVRAIPIGRFAEGAGLTLLIDGAVMPLPDRL
GWEHG