Protein Info for GFF3237 in Sphingobium sp. HT1-2

Annotation: Pyrophosphate-energized proton pump (EC 3.6.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 707 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 54 to 97 (44 residues), see Phobius details amino acids 119 to 146 (28 residues), see Phobius details amino acids 158 to 177 (20 residues), see Phobius details amino acids 234 to 252 (19 residues), see Phobius details amino acids 261 to 283 (23 residues), see Phobius details amino acids 295 to 317 (23 residues), see Phobius details amino acids 338 to 361 (24 residues), see Phobius details amino acids 384 to 410 (27 residues), see Phobius details amino acids 415 to 436 (22 residues), see Phobius details amino acids 478 to 496 (19 residues), see Phobius details amino acids 516 to 536 (21 residues), see Phobius details amino acids 589 to 610 (22 residues), see Phobius details amino acids 616 to 638 (23 residues), see Phobius details amino acids 688 to 705 (18 residues), see Phobius details TIGR01104: V-type H(+)-translocating pyrophosphatase" amino acids 8 to 705 (698 residues), 865.8 bits, see alignment E=1.4e-264 PF03030: H_PPase" amino acids 12 to 699 (688 residues), 872.9 bits, see alignment E=8.9e-267

Best Hits

Swiss-Prot: 65% identical to HPPA_AGRFC: K(+)-insensitive pyrophosphate-energized proton pump (hppA) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01507, inorganic pyrophosphatase [EC: 3.6.1.1] (inferred from 94% identity to sch:Sphch_2386)

Predicted SEED Role

"Pyrophosphate-energized proton pump (EC 3.6.1.1)" in subsystem Phosphate metabolism (EC 3.6.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.1

Use Curated BLAST to search for 3.6.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (707 amino acids)

>GFF3237 Pyrophosphate-energized proton pump (EC 3.6.1.1) (Sphingobium sp. HT1-2)
MQIVHIAIGCGLLAVLYGLFTSRQVLAAPAGNETMQAIAGAIQEGAKAYLGRQYTTIAVV
GIVVAVLVFLFLGITSAVGFLIGAILSGAAGFIGMNISVRANVRTAEASRGTLQSGLTVA
FRAGAITGMLVAGLALLAISVFFWYLTGPAGHAPDDRLVIDSLVALAFGASLISIFARLG
GGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDCAGMAADLFETYVVTVG
ATMVLTALLVTGVDNAFLMRLMALPLIVGGVCIITSIIGTYMVRLGASQSIMGALYKGFW
TTAILSIPALWFVTHYTLGDLDTPFGTDLDGVGGYTGMALFWSMMVGLAVTGLLVVITEY
YTGTNYRPVRSIAKASETGHGTNVIQGLAISLESTALPTIVIVIGIIVAYQLAGLIGVAF
AATAMLALAGMVVALDAYGPVTDNAGGIAEMAHLDDSVRVKTDALDAVGNTTKAVTKGYA
IGSAGLAALVLFGAYTEDLKFYNSALGLTEPVDFSLSNPYVIVGLLLGALLPYLFGAMGM
TAVGRAAGDVVKDVRDQFATNKGIMEGTSRPNYARTVDLVTRAAIKEMIIPSLLPVLAPI
AVYFIIAAVAGVPNGFAALGALLLGVIVSGLFVALSMTSGGGAWDNAKKYIEDGHHGGKG
SEAHKAAVTGDTVGDPYKDTAGPAVNPMIKITNIVALLLLAALAHGA