Protein Info for GFF3231 in Sphingobium sp. HT1-2

Annotation: Transposase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 274 PF13276: HTH_21" amino acids 27 to 82 (56 residues), 52.2 bits, see alignment E=1.2e-17 PF00665: rve" amino acids 108 to 205 (98 residues), 103.9 bits, see alignment E=9.8e-34 PF13683: rve_3" amino acids 195 to 250 (56 residues), 29 bits, see alignment E=1.4e-10 PF13333: rve_2" amino acids 212 to 267 (56 residues), 51.1 bits, see alignment E=2.3e-17

Best Hits

Swiss-Prot: 42% identical to INSF5_ECOLI: Transposase InsF for insertion sequence IS3E (insF5) from Escherichia coli (strain K12)

KEGG orthology group: K07497, putative transposase (inferred from 84% identity to pgv:SL003B_0525)

Predicted SEED Role

"Mobile element protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (274 amino acids)

>GFF3231 Transposase (Sphingobium sp. HT1-2)
MCRCLRIQPSGFYAWQKSPLSTRAQEDARQTELIRQAWNDSGKVYGYRKLHDDLLDQGET
CCPNRVARLTRLAGIKAQVGYKRRPGSYGGKPSLAVDNTLDRQFDVAAPDRAWVTDITYI
RTLEGFAYLAVVIDLYSRRVVGWSMQSRQTTDAVLQALHMAVWRRKPKQRVLIHSDQGSQ
FTSMDWAAFIRAHNLEHSMSRRGNCHDNAVAESFFSSLKRERIRRRTYKTRDEARQDVFD
YIEMFYNPVRKQIRNGMLSPNQFERQQILKAEGV