Protein Info for GFF3209 in Sphingobium sp. HT1-2

Annotation: Dethiobiotin synthase BioD (EC 6.3.3.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13500: AAA_26
  • amino acids 3 to 190 (188 residues), 203.4 bits, E=1.8e-64
• TIGR00347: dethiobiotin synthase
  • amino acids 5 to 156 (152 residues), 112.2 bits, E=1.4e-36

Best Hits

Swiss-Prot: 63% identical to BIOD_RHOPB: ATP-dependent dethiobiotin synthetase BioD (bioD) from Rhodopseudomonas palustris (strain BisB18)

KEGG orthology group: K01935, dethiobiotin synthetase [EC: 6.3.3.3] (inferred from 79% identity to sjp:SJA_C1-02720)

Predicted SEED Role

"Dethiobiotin synthetase (EC 6.3.3.3)" in subsystem Biotin biosynthesis (EC 6.3.3.3)

MetaCyc Pathways

• biotin biosynthesis I (13/15 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate II (3/4 steps found)
• biotin biosynthesis II (4/6 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate III (3/5 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.3.3

Use Curated BLAST to search for 6.3.3.3

Protein Sequence (210 amino acids)

>GFF3209 Dethiobiotin synthase BioD (EC 6.3.3.3) (Sphingobium sp. HT1-2)
MTRLIVTGTDTDIGKTVFAAGLAAALGAYYWKPIQAGVDPQGDKERVAALSGLPPERILP
EAYRLATPASPHLAARIDGVDISLDRLALPRVDGPLVVEGAGGVLVPVSESLLMADLFAY
WGQPAILCARTGLGTINHSLLSIEALRARGVHVVGIAFIGDAHEENERIIPHIAGVPSLG
RLPLLDPLDRDSLAMAFRAHIHLPDNFNGR